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Multiple-step chromosomal integration of divided segments from a large DNA fragment via CRISPR/Cas9 in Escherichia coli.
Li, Yanjun; Yan, Fangqing; Wu, Heyun; Li, Guoliang; Han, Yakun; Ma, Qian; Fan, Xiaoguang; Zhang, Chenglin; Xu, Qingyang; Xie, Xixian; Chen, Ning.
Affiliation
  • Li Y; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
  • Yan F; National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin University of Science and Technology, Tianjin, 300457, China.
  • Wu H; Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China.
  • Li G; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
  • Han Y; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
  • Ma Q; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
  • Fan X; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
  • Zhang C; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
  • Xu Q; National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin University of Science and Technology, Tianjin, 300457, China.
  • Xie X; Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China.
  • Chen N; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
J Ind Microbiol Biotechnol ; 46(1): 81-90, 2019 Jan.
Article in En | MEDLINE | ID: mdl-30470963
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA, Bacterial / Chromosomes, Bacterial / Escherichia coli / DNA Fragmentation / CRISPR-Cas Systems Language: En Journal: J Ind Microbiol Biotechnol Journal subject: BIOTECNOLOGIA / MICROBIOLOGIA Year: 2019 Document type: Article Affiliation country:
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA, Bacterial / Chromosomes, Bacterial / Escherichia coli / DNA Fragmentation / CRISPR-Cas Systems Language: En Journal: J Ind Microbiol Biotechnol Journal subject: BIOTECNOLOGIA / MICROBIOLOGIA Year: 2019 Document type: Article Affiliation country: