Linking single-cell measurements of mass, growth rate, and gene expression.
Genome Biol
; 19(1): 207, 2018 11 27.
Article
in En
| MEDLINE
| ID: mdl-30482222
ABSTRACT
Mass and growth rate are highly integrative measures of cell physiology not discernable via genomic measurements. Here, we introduce a microfluidic platform enabling direct measurement of single-cell mass and growth rate upstream of highly multiplexed single-cell profiling such as single-cell RNA sequencing. We resolve transcriptional signatures associated with single-cell mass and growth rate in L1210 and FL5.12 cell lines and activated CD8+ T cells. Further, we demonstrate a framework using these linked measurements to characterize biophysical heterogeneity in a patient-derived glioblastoma cell line with and without drug treatment. Our results highlight the value of coupled phenotypic metrics in guiding single-cell genomics.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Genomics
/
Microfluidic Analytical Techniques
/
Cell Enlargement
/
Single-Cell Analysis
Type of study:
Evaluation_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Genome Biol
Journal subject:
BIOLOGIA MOLECULAR
/
GENETICA
Year:
2018
Document type:
Article
Affiliation country: