Lower follicular n-3 polyunsaturated fatty acid levels are associated with a better response to ovarian stimulation.
J Assist Reprod Genet
; 36(3): 473-482, 2019 Mar.
Article
in En
| MEDLINE
| ID: mdl-30547270
ABSTRACT
OBJECTIVES:
To analyze in detail the fatty acid (FA) composition of follicular fluid (FF) from two-sized follicles at oocyte retrieval and to determine associations of the FAs from large follicles with the woman's age and the response to ovarian stimulation.DESIGN:
Observational study.SETTING:
University and fertility clinic. PATIENTS Sixty-four women (age 19-46), consisting of unfertile patients and oocyte donors, undergoing controlled ovarian stimulation.INTERVENTIONS:
None. MAIN OUTCOME MEASURE(S) FF from small (< 12 mm) and large (≥ 18 mm) follicles was collected at oocyte retrieval. FAs by gas chromatography-flame ionization detection.RESULT:
Thirty-two FAs with chain lengths ranging from 14 to 25 carbons were identified. There was a readjustment in FA distribution as follicle size increased, raising very long-chain saturated FAs, nervonic (241n-9), arachidonic (204n-6), and n-3 polyunsaturated FAs (PUFA, P < 0.001), the latter mainly due to an increase in docosahexaenoic acid (226n-3, DHA). In large follicles, double bond and peroxidizability indices and total n-3 PUFA, particularly DHA, correlated positively with the woman's age and negatively with the number of total and mature oocytes, total and top-quality embryos, and fertilization rate.CONCLUSIONS:
We have described 32 FAs in ovarian FF, of which 16 changed their distribution with follicle size. The results also indicate that lower n-3 PUFA levels in large follicles, which are associated with younger women, predict a better response to ovarian stimulation based on the recovery of total and mature oocytes, total and top-quality embryos, and fertilization rate per cycle. KEY MESSAGE The fatty acid profile of ovarian FF changes as the follicle grows and lower n-3 PUFA levels in large follicles, associated with younger women, predict a better response to ovarian stimulation.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oocytes
/
Ovulation Induction
/
Fatty Acids, Omega-3
/
Ovarian Follicle
Type of study:
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limits:
Adult
/
Female
/
Humans
Language:
En
Journal:
J Assist Reprod Genet
Journal subject:
GENETICA
/
MEDICINA REPRODUTIVA
Year:
2019
Document type:
Article
Affiliation country: