Effects of separate or combined exposure of nonylphenol and octylphenol on central 5-HT system and related learning and memory in the rats.
Ecotoxicol Environ Saf
; 172: 523-529, 2019 May 15.
Article
in En
| MEDLINE
| ID: mdl-30743168
ABSTRACT
This study evaluated toxic effects of nonylphenol (NP) and octylphenol (OP) on central 5-hydroxytryptamine (5-HT) system and related learning and memory in the rats. Male Sprague-Dawley rats were exposed to NP (30, 90, or 270â¯mg/kg), OP (40, 120, or 360â¯mg/kg), or a mixture of NP and OP [(mixed with the corresponding NP, OP alone exposed low, medium and high dose according to the natural environment exists NPOP = 41; NOL (24â¯mg/kg NP+8â¯mg/kg OP), NOM (72â¯mg/kg NP+24â¯mg/kg OP), NOH (216â¯mg/kg NP+72â¯mg/kg OP)] by gavage every other day for 30 d. Learning and memory were assessed using a passive-avoidance test. Levels of estrogen receptor ß (ERß), 5-HT, tryptophan hydroxylase 2 (TPH2), monoamine oxidase (MAOA) enzyme, serotonin transporter (SERT), the vesicular monoamine transporter 2 (VMAT2), 5-hydroxytryptamine 1â¯A (5-HT1A), 5-hydroxytryptamine 3â¯A (5-HT3A), 5-hydroxytryptamine 3B (5-HT3B), 5-hydroxytryptamine 4â¯A (5-HT4A) and 5-hydroxytryptamine 6â¯A (5-HT6A) were measured using ELISA kits. Levels of ERß, MAOA, SERT, VMAT2, 5-HT1A, 5-HT3A, 5-HT3B, 5-HT4A and 5-HT6A in rat hippocampal reduced by a high dose of NP and/or OP. Levels of TPH2 in rat midbrain and 5-HT in rat hippocampal increased by a high dose of NP and/or OP. In addition, latency was significantly shorter and errors were significantly greater in the high dose NP and NP+OP (NO) groups. Taken together, these results suggest that NP and/or OP may affect learning and memory in rats by inhibiting levels of ERß, which could then lead to decreases in levels of 5-HT1A, 5-HT3A, 5-HT3B, 5-HT4A, and 5-HT6A in the rat hippocampus. These findings suggested that separate and combined exposure to NP and OP could produce toxic effects on central 5-HT system and related learning and memory in the rats.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phenols
/
Serotonin
/
Learning
/
Memory
Limits:
Animals
Language:
En
Journal:
Ecotoxicol Environ Saf
Year:
2019
Document type:
Article