Paternal exposure to benzo(a)pyrene induces genome-wide mutations in mouse offspring.
Commun Biol
; 2: 228, 2019.
Article
in En
| MEDLINE
| ID: mdl-31240266
ABSTRACT
Understanding the effects of environmental exposures on germline mutation rates has been a decades-long pursuit in genetics. We used next-generation sequencing and comparative genomic hybridization arrays to investigate genome-wide mutations in the offspring of male mice exposed to benzo(a)pyrene (BaP), a common environmental pollutant. We demonstrate that offspring developing from sperm exposed during the mitotic or post-mitotic phases of spermatogenesis have significantly more de novo single nucleotide variants (1.8-fold; P < 0.01) than controls. Both phases of spermatogenesis are susceptible to the induction of heritable mutations, although mutations arising from post-fertilization events are more common after post-mitotic exposure. In addition, the mutation spectra in sperm and offspring of BaP-exposed males are consistent. Finally, we report a significant increase in transmitted copy number duplications (P = 0.001) in BaP-exposed sires. Our study demonstrates that germ cell mutagen exposures induce genome-wide mutations in the offspring that may be associated with adverse health outcomes.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spermatozoa
/
Benzo(a)pyrene
/
Paternal Exposure
/
Environmental Pollutants
/
Mutagens
/
Mutation
Limits:
Animals
Language:
En
Journal:
Commun Biol
Year:
2019
Document type:
Article