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m5C Methylation Guides Systemic Transport of Messenger RNA over Graft Junctions in Plants.
Yang, Lei; Perrera, Valentina; Saplaoura, Eleftheria; Apelt, Federico; Bahin, Mathieu; Kramdi, Amira; Olas, Justyna; Mueller-Roeber, Bernd; Sokolowska, Ewelina; Zhang, Wenna; Li, Runsheng; Pitzalis, Nicolas; Heinlein, Manfred; Zhang, Shoudong; Genovesio, Auguste; Colot, Vincent; Kragler, Friedrich.
Affiliation
  • Yang L; Max-Planck-Institute of Molecular Plant Physiology, Wissenschaftspark Golm, Am Mühlenberg 1, 14476 Golm, Germany.
  • Perrera V; Institut de Biologie de l'Ecole Normale Supérieure (IBENS), CNRS UMR8197, INSERM U1024, PSL Research University, 75230 Paris, France.
  • Saplaoura E; Max-Planck-Institute of Molecular Plant Physiology, Wissenschaftspark Golm, Am Mühlenberg 1, 14476 Golm, Germany.
  • Apelt F; Max-Planck-Institute of Molecular Plant Physiology, Wissenschaftspark Golm, Am Mühlenberg 1, 14476 Golm, Germany.
  • Bahin M; Institut de Biologie de l'Ecole Normale Supérieure (IBENS), CNRS UMR8197, INSERM U1024, PSL Research University, 75230 Paris, France.
  • Kramdi A; Institut de Biologie de l'Ecole Normale Supérieure (IBENS), CNRS UMR8197, INSERM U1024, PSL Research University, 75230 Paris, France.
  • Olas J; Institute of Biochemistry and Biology, University of Potsdam, Department of Molecular Biology, Karl-Liebknecht-Strasse 24-25, Haus 20, 14476 Potsdam, Germany.
  • Mueller-Roeber B; Institute of Biochemistry and Biology, University of Potsdam, Department of Molecular Biology, Karl-Liebknecht-Strasse 24-25, Haus 20, 14476 Potsdam, Germany.
  • Sokolowska E; Max-Planck-Institute of Molecular Plant Physiology, Wissenschaftspark Golm, Am Mühlenberg 1, 14476 Golm, Germany.
  • Zhang W; Max-Planck-Institute of Molecular Plant Physiology, Wissenschaftspark Golm, Am Mühlenberg 1, 14476 Golm, Germany; China Agricultural University, 17 Qinghua East Road, 100080 Haidian, Beijing, China.
  • Li R; Department of Biology, Hong Kong Baptist University, Hong Kong, Hong Kong SAR, China.
  • Pitzalis N; Institut de Biologie Moléculaire des Plantes du CNRS, IBMP-CNRS UPR2357, 12, rue du Général Zimmer, 67084 Strasbourg, France.
  • Heinlein M; Institut de Biologie Moléculaire des Plantes du CNRS, IBMP-CNRS UPR2357, 12, rue du Général Zimmer, 67084 Strasbourg, France.
  • Zhang S; Department of Biology, Hong Kong Baptist University, Hong Kong, Hong Kong SAR, China; Centre for Soybean Research, Partner State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, Special Administrative Region, China.
  • Genovesio A; Institut de Biologie de l'Ecole Normale Supérieure (IBENS), CNRS UMR8197, INSERM U1024, PSL Research University, 75230 Paris, France.
  • Colot V; Institut de Biologie de l'Ecole Normale Supérieure (IBENS), CNRS UMR8197, INSERM U1024, PSL Research University, 75230 Paris, France.
  • Kragler F; Max-Planck-Institute of Molecular Plant Physiology, Wissenschaftspark Golm, Am Mühlenberg 1, 14476 Golm, Germany. Electronic address: kragler@mpimp-golm.mpg.de.
Curr Biol ; 29(15): 2465-2476.e5, 2019 08 05.
Article in En | MEDLINE | ID: mdl-31327714
ABSTRACT
In plants, transcripts move to distant body parts to potentially act as systemic signals regulating development and growth. Thousands of messenger RNAs (mRNAs) are transported across graft junctions via the phloem to distinct plant parts. Little is known regarding features, structural motifs, and potential base modifications of transported transcripts and how these may affect their mobility. We identified Arabidopsis thaliana mRNAs harboring the modified base 5-methylcytosine (m5C) and found that these are significantly enriched in mRNAs previously described as mobile, moving over graft junctions to distinct plant parts. We confirm this finding with graft-mobile methylated mRNAs TRANSLATIONALLY CONTROLLED TUMOR PROTEIN 1 (TCTP1) and HEAT SHOCK COGNATE PROTEIN 70.1 (HSC70.1), whose mRNA transport is diminished in mutants deficient in m5C mRNA methylation. Together, our results point toward an essential role of cytosine methylation in systemic mRNA mobility in plants and that TCTP1 mRNA mobility is required for its signaling function.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA, Messenger / Arabidopsis / RNA, Plant / HSP70 Heat-Shock Proteins / Arabidopsis Proteins / 5-Methylcytosine / Microtubule-Associated Proteins Type of study: Prognostic_studies Language: En Journal: Curr Biol Journal subject: BIOLOGIA Year: 2019 Document type: Article Affiliation country:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA, Messenger / Arabidopsis / RNA, Plant / HSP70 Heat-Shock Proteins / Arabidopsis Proteins / 5-Methylcytosine / Microtubule-Associated Proteins Type of study: Prognostic_studies Language: En Journal: Curr Biol Journal subject: BIOLOGIA Year: 2019 Document type: Article Affiliation country: