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Versatile cell ablation tools and their applications to study loss of cell functions.
Liu, Fengming; Dai, Shen; Feng, Dechun; Peng, Xiao; Qin, Zhongnan; Kearns, Alison C; Huang, Wenfei; Chen, Yong; Ergün, Süleyman; Wang, Hong; Rappaport, Jay; Bryda, Elizabeth C; Chandrasekhar, Anand; Aktas, Bertal; Hu, Hongzhen; Chang, Sulie L; Gao, Bin; Qin, Xuebin.
Affiliation
  • Liu F; Department of Neuroscience, Lewis Katz School of Medicine at Temple University, 3500 N Broad Street, Philadelphia, PA, 19140, USA.
  • Dai S; Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA, 70433, USA.
  • Feng D; Department of Immunology and Microbiology, Tulane University School of Medicine, New Orleans, LA, 70112, USA.
  • Peng X; Department of Neuroscience, Lewis Katz School of Medicine at Temple University, 3500 N Broad Street, Philadelphia, PA, 19140, USA.
  • Qin Z; Laboratory of Liver Diseases, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD, 20892, USA.
  • Kearns AC; Department of Neuroscience, Lewis Katz School of Medicine at Temple University, 3500 N Broad Street, Philadelphia, PA, 19140, USA.
  • Huang W; Department of Neuroscience, Lewis Katz School of Medicine at Temple University, 3500 N Broad Street, Philadelphia, PA, 19140, USA.
  • Chen Y; Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA, 70433, USA.
  • Ergün S; Department of Immunology and Microbiology, Tulane University School of Medicine, New Orleans, LA, 70112, USA.
  • Wang H; Department of Neuroscience, Lewis Katz School of Medicine at Temple University, 3500 N Broad Street, Philadelphia, PA, 19140, USA.
  • Rappaport J; Institute of NeuroImmune Pharmacology, Seton Hall University, 400 South Orange Avenue, South Orange, NJ, 07079, USA.
  • Bryda EC; Department of Neuroscience, Lewis Katz School of Medicine at Temple University, 3500 N Broad Street, Philadelphia, PA, 19140, USA.
  • Chandrasekhar A; Key Lab for Immunology in Universities of Shandong Province, School of Clinical Medicine, Weifang Medical University, 261053, Weifang, People's Republic of China.
  • Aktas B; Institute of Anatomy and Cell Biology, Julius-Maximillan University, 97070, Wurzburg, Germany.
  • Hu H; Department of Neuroscience, Lewis Katz School of Medicine at Temple University, 3500 N Broad Street, Philadelphia, PA, 19140, USA.
  • Chang SL; Division of Pathology, Tulane National Primate Research Center, 18703 Three Rivers Road, Covington, LA, 70433, USA.
  • Gao B; Rat Resource and Research Center, University of Missouri, 4011 Discovery Drive, Columbia, MO, 65201, USA.
  • Qin X; Division of Biological Sciences, 340D Life Sciences Center, University of Missouri, 1201 Rollins St, Columbia, MO, USA.
Cell Mol Life Sci ; 76(23): 4725-4743, 2019 Dec.
Article in En | MEDLINE | ID: mdl-31359086
ABSTRACT
Targeted cell ablation is a powerful approach for studying the role of specific cell populations in a variety of organotypic functions, including cell differentiation, and organ generation and regeneration. Emerging tools for permanently or conditionally ablating targeted cell populations and transiently inhibiting neuronal activities exhibit a diversity of application and utility. Each tool has distinct features, and none can be universally applied to study different cell types in various tissue compartments. Although these tools have been developed for over 30 years, they require additional improvement. Currently, there is no consensus on how to select the tools to answer the specific scientific questions of interest. Selecting the appropriate cell ablation technique to study the function of a targeted cell population is less straightforward than selecting the method to study a gene's functions. In this review, we discuss the features of the various tools for targeted cell ablation and provide recommendations for optimal application of specific approaches.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacteriocins / Clodronic Acid / Simplexvirus / Diphtheria Toxin / Optogenetics Limits: Animals / Humans Language: En Journal: Cell Mol Life Sci Journal subject: BIOLOGIA MOLECULAR Year: 2019 Document type: Article Affiliation country:
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacteriocins / Clodronic Acid / Simplexvirus / Diphtheria Toxin / Optogenetics Limits: Animals / Humans Language: En Journal: Cell Mol Life Sci Journal subject: BIOLOGIA MOLECULAR Year: 2019 Document type: Article Affiliation country: