Effects of collagenase type I on the structural features of collagen fibres from sea cucumber (Stichopus japonicus) body wall.
Food Chem
; 301: 125302, 2019 Dec 15.
Article
in En
| MEDLINE
| ID: mdl-31387034
ABSTRACT
The autolysis of sea cucumber is caused by depolymerisation of collagen fibres and unfolding of fibrils. In order to highlight the role of collagenase in sea cucumber autolysis, collagen fibres from sea cucumber were hydrolysed with collagenase type I. Electron microscopy (EM) results indicated the collagenase caused partial depolymerisation of collagen fibres into fibrils due to the fracture of proteoglycan interfibrillar bridges, as well as uncoiling of collagen fibrils. Chemical analysis and SDS-PAGE both indicated collagenase induced a time-dependent release of glycosaminoglycans (GAGs) and soluble proteins, which further demonstrated the degradation of proteoglycan interfibrillar bridges. Collagenase also degraded collagens by releasing soluble hydroxyproline (Hpy), with the dissolution rate of Hyp reaching 11.11% after 72â¯h. Fourier transform infrared analysis showed that collagenase caused the reduction of intermolecular interactions and structural order of collagen. Hence, collagenase participated in the autolysis of sea cucumber by deteriorating both macromolecular and monomeric collagens.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Collagen
/
Collagenases
/
Stichopus
Limits:
Animals
Language:
En
Journal:
Food Chem
Year:
2019
Document type:
Article