Akt phosphorylation of mitochondrial Lonp1 protease enables oxidative metabolism and advanced tumor traits.
Oncogene
; 38(43): 6926-6939, 2019 10.
Article
in En
| MEDLINE
| ID: mdl-31406245
ABSTRACT
Tumor mitochondria have heightened protein folding quality control, but the regulators of this process and how they impact cancer traits are not completely understood. Here we show that the ATP-directed mitochondrial protease, LonP1 is upregulated by stress conditions, including hypoxia, in tumor, but not normal cells. In mitochondria, LonP1 is phosphorylated by Akt on Ser173 and Ser181, enhancing its protease activity. Interference with this pathway induces accumulation of misfolded subunits of electron transport chain complex II and complex V, resulting in impaired oxidative bioenergetics and heightened ROS production. Functionally, this suppresses mitochondrial trafficking to the cortical cytoskeleton, shuts off tumor cell migration and invasion, and inhibits primary and metastatic tumor growth, in vivo. These data identify LonP1 as a key effector of mitochondrial reprogramming in cancer and potential therapeutic target.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phosphorylation
/
Mitochondrial Proteins
/
ATP-Dependent Proteases
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Proto-Oncogene Proteins c-akt
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Mitochondria
/
Neoplasms
Limits:
Animals
/
Humans
Language:
En
Journal:
Oncogene
Journal subject:
BIOLOGIA MOLECULAR
/
NEOPLASIAS
Year:
2019
Document type:
Article
Affiliation country: