Upregulated microRNA6713p promotes tumor progression by suppressing forkhead box P2 expression in nonsmallcell lung cancer.
Mol Med Rep
; 20(4): 3149-3159, 2019 Oct.
Article
in En
| MEDLINE
| ID: mdl-31432170
ABSTRACT
In the present study, the expression of microRNA (miR)6713p in nonsmallcell lung cancer (NSCLC) was detected via reverse transcriptionquantitative polymerase chain reaction analysis, and its role in cell proliferation, apoptosis, migration and invasion was investigated via Cell Counting Kit8, colony formation, flow cytometry, Transwell and scratch assays, respectively. It was observed that the expression of miR6713p was upregulated in NSCLC tissues and cell lines (A549 and H1975). Treatment with miR6713p inhibitors suppressed cell proliferation, migration and invasion, and increased apoptosis in vitro, suggesting that miR6713p functions as an oncogene in NSCLC. In addition, forkhead box P2 (FOXP2) has been reported to be a tumor suppressor that is downregulated in several types of cancer, and its low expression was confirmed in NSCLC tissues and cell lines in the current study via western blotting. The results of the luciferase reporter assay also demonstrated that miR6713p targeted directly the 3'untranslated region of FOXP2. Furthermore, overexpression of FOXP2 in A549 and H1975 cell lines suppressed the growth, migration and invasion, and promoted apoptosis, whereas these effects were reversed by transfection with miR6713p mimics, suggesting that miR6713p promoted tumor progression via regulating FOXP2. Taken together, the results reported in the present study implied that miR6713p may be a potential therapeutic target in NSCLC.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA, Neoplasm
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Gene Expression Regulation, Neoplastic
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Carcinoma, Non-Small-Cell Lung
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MicroRNAs
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Forkhead Transcription Factors
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Lung Neoplasms
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Neoplasm Proteins
Limits:
Aged
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Female
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Humans
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Male
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Middle aged
Language:
En
Journal:
Mol Med Rep
Year:
2019
Document type:
Article