High serum extracellular vesicle miR-10b expression predicts poor prognosis in patients with acute myeloid leukemia.
Cancer Biomark
; 27(1): 1-9, 2020.
Article
in En
| MEDLINE
| ID: mdl-31594209
ABSTRACT
BACKGROUND:
Increasing evidence have demonstrated that serum extracellular vesicle microRNAs (EV-miRNAs) are promising noninvasive biomarkers for various cancer types.OBJECTIVE:
In this study, we aimed to investigate and evaluate the potential clinical significance of serum EV-miR-10b for acute myeloid leukemia (AML).METHODS:
Blood samples were collected from a cohort of 95 de novo AML patients and 80 healthy individuals. Of all AML patients, 51 patients were considered as cytogenetic normal AML (CN-AML). Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was performed to measure the expression levels of serum EV-miR-10b.RESULTS:
The extracellular vesicles we extracted from the serum samples were positive for EV/exosome markers including TSG101, CD63, Flotillin-1 and CD9. The expression levels of serum EV-miR-10b were significantly higher in AML/CN-AML patients than healthy controls. In addition, serum EV-miR-10b expression was strongly correlated with aggressive clinical characteristics. Moreover, receiver operating characteristic analysis showed serum EV-miR-10b yielded an area under the curve of 0.875, with 77.89% specificity and 82.50% sensitivity in identifying AML patients from normal controls. Furthermore, AML patients with higher serum EV-miR-10b expression had significantly shorter survival and serum EV-miR-10b was demonstrated to be an independent prognostic marker for overall survival in AML.CONCLUSIONS:
Taken together, serum EV-miR-10b might serve as a promising biomarker for predicting the prognosis of AML.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Prognosis
/
Leukemia, Myeloid, Acute
/
Biomarkers, Tumor
/
MicroRNAs
Type of study:
Prognostic_studies
/
Risk_factors_studies
Limits:
Adult
/
Aged
/
Humans
/
Middle aged
Language:
En
Journal:
Cancer Biomark
Journal subject:
BIOQUIMICA
/
NEOPLASIAS
Year:
2020
Document type:
Article