Pesticide-derived bright chlorine-doped carbon dots for selective determination and intracellular imaging of Fe(III).

ABSTRACT

Inspired by the conversion from organics or biomass to fluorescent carbon dots (C-dots), the use of pesticide 4-chlorophenol (4-CP) as a precursor to prepare C-dots has been reported. The as-prepared chlorine-doped C-dots display a brightly blue emission at ∼445 nm with ∼22.8% quantum yield. Also, the surface of C-dots enriches functional groups, such as phenolic hydroxyl and carboxylic acid, etc., which can capture ferric ion (Fe(III)), resulting in the quenching of blue fluorescence of C-dots through an inner filter effect. The quantitative assay for Fe(III) was therefore realized by this probe with a 0.36 µM detection limit in the 0.6-25 µM concentration range. Most significantly, the cytotoxicity on Hela cells indicates the 4-CP-derived C-dots have a negligible cytotoxicity. The C-dots were applied in detection in environmental samples and imaging in Hela cells of Fe(III), demonstrating their good applicability, low toxicity and good biocompatibility, and providing an alterative approach to totally eliminate the harm of chlorophenols (CPs).