Flow Cytometry in the Differential Diagnosis of CD10-Positive Nodal Lymphomas.
Lab Med
; 51(4): 385-393, 2020 Jul 08.
Article
in En
| MEDLINE
| ID: mdl-31834933
ABSTRACT
BACKGROUND:
Differences between follicular lymphoma (FL) and diffuse large B-cell lymphoma/high-grade B-cell lymphoma (DLBCL/HGBL) by flow cytometry are underexplored.METHODS:
We retrospectively assessed flow cytometry results from 191 consecutive lymph node biopsies diagnosed with FL or DLBCL/HGBL.RESULTS:
The only parameters that differed between the 2 groups in the derivation cohort were forward scatter and side scatter (P < 10-6; area under the curve [AUC], 0.75-0.8) and %CD23 (P = .004; area under the receiver characteristic operating curve, 0.64). However, since light scatter characteristics did not distinguish between grade 3 FL and DLBCL/HGBL, we set out to develop a model with high sensitivity for the exclusion of the latter. Several models, including FS and %CD23, were tested, and 2 models showed a sensitivity of >0.90, with negative predictive values of ≥0.95, albeit with low specificity (0.45 to 0.57).CONCLUSION:
Two simple models enable the exclusion of DLBCL/HGBL with a high degree of confidence.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Lymphoma, Follicular
/
Lymphoma, Large B-Cell, Diffuse
/
Flow Cytometry
/
Lymph Nodes
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Lab Med
Year:
2020
Document type:
Article
Affiliation country: