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Profiling the effect of nafcillin on HA-MRSA D712 using bacteriological and physiological media.
Rajput, Akanksha; Poudel, Saugat; Tsunemoto, Hannah; Meehan, Michael; Szubin, Richard; Olson, Connor A; Lamsa, Anne; Seif, Yara; Dillon, Nicholas; Vrbanac, Alison; Sugie, Joseph; Dahesh, Samira; Monk, Jonathan M; Dorrestein, Pieter C; Knight, Rob; Nizet, Victor; Palsson, Bernhard O; Feist, Adam M; Pogliano, Joe.
Affiliation
  • Rajput A; Department of Bioengineering, University of California, San Diego, La Jolla, USA.
  • Poudel S; Department of Bioengineering, University of California, San Diego, La Jolla, USA.
  • Tsunemoto H; Division of Biological Sciences, University of California San Diego, La Jolla, CA, 92093, USA.
  • Meehan M; Collaborative Mass Spectrometry Innovation Center, University of California, San Diego, La Jolla, California, USA.
  • Szubin R; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA.
  • Olson CA; Department of Bioengineering, University of California, San Diego, La Jolla, USA.
  • Lamsa A; Department of Bioengineering, University of California, San Diego, La Jolla, USA.
  • Seif Y; Division of Biological Sciences, University of California San Diego, La Jolla, CA, 92093, USA.
  • Dillon N; Department of Bioengineering, University of California, San Diego, La Jolla, USA.
  • Vrbanac A; Department of Pediatrics, University of California, San Diego, La Jolla, CA, USA.
  • Sugie J; Collaborative to Halt Antibiotic-Resistant Microbes (CHARM), Department of Pediatrics, UC San Diego, La Jolla, CA, 92093, USA.
  • Dahesh S; Department of Pediatrics, University of California, San Diego, La Jolla, CA, USA.
  • Monk JM; Collaborative to Halt Antibiotic-Resistant Microbes (CHARM), Department of Pediatrics, UC San Diego, La Jolla, CA, 92093, USA.
  • Dorrestein PC; Division of Biological Sciences, University of California San Diego, La Jolla, CA, 92093, USA.
  • Knight R; Department of Pediatrics, University of California, San Diego, La Jolla, CA, USA.
  • Nizet V; Collaborative to Halt Antibiotic-Resistant Microbes (CHARM), Department of Pediatrics, UC San Diego, La Jolla, CA, 92093, USA.
  • Palsson BO; Department of Bioengineering, University of California, San Diego, La Jolla, USA.
  • Feist AM; Collaborative Mass Spectrometry Innovation Center, University of California, San Diego, La Jolla, California, USA.
  • Pogliano J; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA.
Sci Data ; 6(1): 322, 2019 12 17.
Article in En | MEDLINE | ID: mdl-31848353
ABSTRACT
Staphylococcus aureus strains have been continuously evolving resistance to numerous classes of antibiotics including methicillin, vancomycin, daptomycin and linezolid, compounding the enormous healthcare and economic burden of the pathogen. Cation-adjusted Mueller-Hinton broth (CA-MHB) is the standard bacteriological media for measuring antibiotic susceptibility in the clinical lab, but the use of media that more closely mimic the physiological state of the patient, e.g. mammalian tissue culture media, can in certain circumstances reveal antibiotic activities that may be more predictive of effectiveness in vivo. In the current study, we use both types of media to explore antibiotic resistance phenomena in hospital-acquired USA100 lineage methicillin-resistant, vancomycin-intermediate Staphylococcus aureus (MRSA/VISA) strain D712 via multidimensional high throughput analysis of growth rates, bacterial cytological profiling, RNA sequencing, and exo-metabolomics (HPLC and LC-MS). Here, we share data generated from these assays to shed light on the antibiotic resistance behavior of MRSA/VISA D712 in both bacteriological and physiological media.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Drug Resistance, Multiple, Bacterial / Methicillin-Resistant Staphylococcus aureus / Nafcillin Language: En Journal: Sci Data Year: 2019 Document type: Article Affiliation country:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Drug Resistance, Multiple, Bacterial / Methicillin-Resistant Staphylococcus aureus / Nafcillin Language: En Journal: Sci Data Year: 2019 Document type: Article Affiliation country: