Assessment of NK cell-mediated cytotoxicity by flow cytometry after rapid, high-yield isolation from peripheral blood.
Methods Enzymol
; 631: 277-287, 2020.
Article
in En
| MEDLINE
| ID: mdl-31948552
ABSTRACT
Natural killer (NK) cells constitute the predominant innate lymphocyte subset that mediates the anti-viral and anti-tumor immune responses. NK cells use an array of innate receptors to sense their environment and to respond to infections, cellular stress and transformation. The resulting NK cell activation, including cytotoxicity and cytokine production, is a fundamental component of the early immune response. The most recent discoveries in NK cell biology have stimulated the translational research that has led to remarkable results for the treatment of human malignancies. Therefore, the rapid isolation of NK cells from the peripheral blood or tumor microenvironment and the subsequent assessment of cytolytic function are crucial to the study of their potency and NK cell-mediated immunosurveillance. Here, we provide protocols for NK cell isolation and the assessment of NK cell cytotoxicity using flow cytometry.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cytotoxicity Tests, Immunologic
/
Killer Cells, Natural
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Cytotoxicity, Immunologic
/
Flow Cytometry
Type of study:
Guideline
Limits:
Humans
Language:
En
Journal:
Methods Enzymol
Year:
2020
Document type:
Article
Affiliation country: