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Quantitative Proteomics of the Cancer Cell Line Encyclopedia.
Nusinow, David P; Szpyt, John; Ghandi, Mahmoud; Rose, Christopher M; McDonald, E Robert; Kalocsay, Marian; Jané-Valbuena, Judit; Gelfand, Ellen; Schweppe, Devin K; Jedrychowski, Mark; Golji, Javad; Porter, Dale A; Rejtar, Tomas; Wang, Y Karen; Kryukov, Gregory V; Stegmeier, Frank; Erickson, Brian K; Garraway, Levi A; Sellers, William R; Gygi, Steven P.
Affiliation
  • Nusinow DP; Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA. Electronic address: david_nusinow@hms.harvard.edu.
  • Szpyt J; Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
  • Ghandi M; Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
  • Rose CM; Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
  • McDonald ER; Novartis Institutes for Biomedical Research, Cambridge, MA 02139, USA.
  • Kalocsay M; Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
  • Jané-Valbuena J; Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
  • Gelfand E; Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
  • Schweppe DK; Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
  • Jedrychowski M; Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
  • Golji J; Novartis Institutes for Biomedical Research, Cambridge, MA 02139, USA.
  • Porter DA; Novartis Institutes for Biomedical Research, Cambridge, MA 02139, USA.
  • Rejtar T; Novartis Institutes for Biomedical Research, Cambridge, MA 02139, USA.
  • Wang YK; Novartis Institutes for Biomedical Research, Cambridge, MA 02139, USA.
  • Kryukov GV; Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
  • Stegmeier F; Novartis Institutes for Biomedical Research, Cambridge, MA 02139, USA.
  • Erickson BK; Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
  • Garraway LA; Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA; Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA.
  • Sellers WR; Novartis Institutes for Biomedical Research, Cambridge, MA 02139, USA.
  • Gygi SP; Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA. Electronic address: steven_gygi@hms.harvard.edu.
Cell ; 180(2): 387-402.e16, 2020 01 23.
Article in En | MEDLINE | ID: mdl-31978347
ABSTRACT
Proteins are essential agents of biological processes. To date, large-scale profiling of cell line collections including the Cancer Cell Line Encyclopedia (CCLE) has focused primarily on genetic information whereas deep interrogation of the proteome has remained out of reach. Here, we expand the CCLE through quantitative profiling of thousands of proteins by mass spectrometry across 375 cell lines from diverse lineages to reveal information undiscovered by DNA and RNA methods. We observe unexpected correlations within and between pathways that are largely absent from RNA. An analysis of microsatellite instable (MSI) cell lines reveals the dysregulation of specific protein complexes associated with surveillance of mutation and translation. These and other protein complexes were associated with sensitivity to knockdown of several different genes. These data in conjunction with the wider CCLE are a broad resource to explore cellular behavior and facilitate cancer research.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Gene Expression Regulation, Neoplastic / Proteome / Neoplasms Limits: Humans Language: En Journal: Cell Year: 2020 Document type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Gene Expression Regulation, Neoplastic / Proteome / Neoplasms Limits: Humans Language: En Journal: Cell Year: 2020 Document type: Article