Subcellular Chemical Imaging: New Avenues in Cell Biology.
Trends Cell Biol
; 30(3): 173-188, 2020 03.
Article
in En
| MEDLINE
| ID: mdl-31987730
ABSTRACT
To better understand the physiology and acclimation capability of the cell, one of the great challenges of the future is to access the interior of a cell and unveil its chemical landscape (composition and distribution of elements and molecules). Chemical imaging has greatly improved in sensitivity and spatial resolution to visualize and quantify nutrients, metabolites, toxic elements, and drugs in single cells at the subcellular level. This review aims to present the current potential of these emerging imaging technologies and to guide biologists towards a strategy for interrogating biological processes at the nanoscale. We also describe various solutions to combine multiple imaging techniques in a correlative way and provide perspectives and future directions for integrative subcellular imaging across different disciplines.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cells
/
Imaging, Three-Dimensional
/
Cell Biology
Limits:
Animals
/
Humans
Language:
En
Journal:
Trends Cell Biol
Year:
2020
Document type:
Article