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Aligned soy protein isolate-modified poly(L-lactic acid) nanofibrous conduits enhanced peripheral nerve regeneration.
Zhang, Qiang; Tong, Zan; Chen, Feixiang; Wang, Xiaomei; Ren, Mingxin; Zhao, Yanan; Wu, Ping; He, Xiaohua; Chen, Pu; Chen, Yun.
Affiliation
  • Zhang Q; Department of Biomedical Engineering and Hubei Province Key Laboratory of Allergy and Immune Related Diseases, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, People's Republic of China. Hangzhou Singclean Medical Products Co., Ltd., Hangzhou 310018, People's Republic of China.
J Neural Eng ; 17(3): 036003, 2020 06 02.
Article in En | MEDLINE | ID: mdl-32340001
ABSTRACT

OBJECTIVE:

Repair and regeneration of peripheral nerve defect by engineered conduits have greatly advanced in the past decades while still facing great challenges.

APPROACH:

In this work, we fabricated a new highly oriented poly(L-lactic acid) (PLLA)/soy protein isolate (SPI) nanofibrous conduit (HO-PSNC) for nerve regeneration. MAIN

RESULTS:

Firstly, we observed that SPI could efficiently modify PLLA for the electrospinning of PLLA/SPI nanofibers with enhanced physical and biological properties. Incorporation of SPI decreased the fiber diameter and ductility of PLLA/SPI nanofibrous films (PSNFs), improved the tensile strength and surface wettability of PSNFs and increased the in vivo degradability of the PSNFs. When the hybrid ratio of SPI was 20 and 40%, PSNFs could efficiently promote neural cell extension and differentiation in vitro. Based on these data, 20% SPI (PSNF-20) was chosen for further investigation. Next, PSNF-20 with different fiber orientations (random/low orientation, medium, and high orientation, respectively) were developed and used for evaluating neural cell behaviors on the materials. Results revealed that the PSNF-20 with highly oriented nanofibers (HO-PSNF-20) or mediumly oriented nanofibers (MO-PSNF-20) showed a better performance in directing cell extension and enhancing neurite outgrowth. Finally, the highly oriented nanofibers conduits (HO-PSNC-20) were used to bridge sciatic nerve defect in rats with highly oriented PLLA and autografts as controls. HO-PSNC-20 exhibited a significant promotion in nerve regeneration and functional reconstruction comparing to highly oriented PLLA as proven by the evaluations of walking track, electrophysiology, toluidine blue nerve staining, transmission electron microscopy, neural factors staining and qPCR, and gastrocnemius histology.

SIGNIFICANCE:

In conclusion, nerve conduit fabricated from aligned electrospinning of SPI-modified PLLA nanofibers is promising for peripheral nerve regeneration.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Nanofibers Limits: Animals Language: En Journal: J Neural Eng Journal subject: NEUROLOGIA Year: 2020 Document type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Nanofibers Limits: Animals Language: En Journal: J Neural Eng Journal subject: NEUROLOGIA Year: 2020 Document type: Article