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Detection of Zika Virus Using Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP).
Lamb, Laura E; Bartolone, Sarah N; Chancellor, Michael B.
Affiliation
  • Lamb LE; Beaumont Health, Royal Oak, MI, USA. Laura.Lamb@Beaumont.Org.
  • Bartolone SN; Oakland University, William Beaumont School of Medicine, Rochester, MI, USA. Laura.Lamb@Beaumont.Org.
  • Chancellor MB; Beaumont Health, Royal Oak, MI, USA.
Methods Mol Biol ; 2142: 137-146, 2020.
Article in En | MEDLINE | ID: mdl-32367365
ABSTRACT
Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) allows amplification and detection of RNA or DNA rapidly and relatively inexpensively. Here we describe how RT-LAMP can be utilized to detect Zika virus in human urine or serum samples or Aedes mosquito samples. This can be completed in under 30 min and without first isolating the RNA from the sample.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Nucleic Acid Amplification Techniques / Molecular Diagnostic Techniques / Zika Virus / Zika Virus Infection Type of study: Diagnostic_studies Limits: Animals / Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2020 Document type: Article Affiliation country:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Nucleic Acid Amplification Techniques / Molecular Diagnostic Techniques / Zika Virus / Zika Virus Infection Type of study: Diagnostic_studies Limits: Animals / Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2020 Document type: Article Affiliation country: