Characterization of carbapenem-resistant Klebsiella pneumoniae in a tertiary hospital in Fuzhou, China.
J Appl Microbiol
; 129(5): 1220-1226, 2020 Nov.
Article
in En
| MEDLINE
| ID: mdl-32396222
ABSTRACT
AIMS:
The emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) strains has led to increased mortality and morbidity rates. Tigecycline, a new class of broad-spectrum glycyl-tetracycline antibiotics, has been used to target multi- and pan-drug-resistant bacterial infections. This study aimed to assess the molecular characteristics of CRKP in a tertiary hospital, and its susceptibility to tigecycline, to create a reference for hospital infection control and clinical drug use. METHODS ANDRESULTS:
We retrieved patient clinical information and CRKP characterization from medical records and detected the MIC of tigecycline using the micro-broth dilution method. Multi-locus sequence typing was performed, and antibiotic resistance genes associated with CRKP were detected by qPCR. A total of 166 CRKP strains were detected in the sputum, urine and blood among intensive care unit patients (average age, 69·6 years). The most infrequently observed resistance genes were amikacin resistance genes, followed by tobramycin resistance genes. KPC-2, CTX-M9 and CTX-M1 were the most frequently detected resistance genes.CONCLUSIONS:
No strain was resistant to tigecycline (MIC ≥ 8 µg ml-1 ). Twenty-four sequence types were identified, with ST11 being the most common type. SIGNIFICANCE AND IMPACT OF THE STUDY Clinicians and infection control experts should be aware of CRKP prevalence to facilitate clinical treatment and improve nosocomial infection control.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Klebsiella Infections
/
Carbapenem-Resistant Enterobacteriaceae
/
Klebsiella pneumoniae
Type of study:
Prognostic_studies
/
Risk_factors_studies
Limits:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
/
Middle aged
Country/Region as subject:
Asia
Language:
En
Journal:
J Appl Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2020
Document type:
Article
Affiliation country: