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Polymerase chain reaction primer sets for the detection of genetically diverse human sapoviruses.
Oka, Tomoichiro; Yamamoto, Seiji P; Iritani, Nobuhiro; Sato, Shigenori; Tatsumi, Chika; Mita, Tetsuo; Yahiro, Shunsuke; Shibata, Shinichiro; Wu, Fang-Tzy; Takagi, Hirotaka.
Affiliation
  • Oka T; Department of Virology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama-shi, Tokyo, 208-0011, Japan. oka-t@nih.go.jp.
  • Yamamoto SP; Division of Microbiology, Osaka Institute of Public Health, Osaka, Japan.
  • Iritani N; Division of Microbiology, Osaka Institute of Public Health, Osaka, Japan.
  • Sato S; Division of Virology and Medical Zoology, Chiba Prefectural Institute of Public Health, Chiba, Japan.
  • Tatsumi C; Division of Virology, Shimane Prefectural Institute of Public Health and Environmental Science, Shimane, Japan.
  • Mita T; Division of Virology, Shimane Prefectural Institute of Public Health and Environmental Science, Shimane, Japan.
  • Yahiro S; Department of Microbiology, Kumamoto Prefectural Institute of Public Health and Environmental Science, Kumamoto, Japan.
  • Shibata S; Microbiology Department, Nagoya City Public Health Research Institute, Aichi, Japan.
  • Wu FT; Center for Research, Diagnostics and Vaccine Development, Taiwan Centers for Disease Control, Taipei, Taiwan.
  • Takagi H; Management Department of Biosafety and Laboratory Animal, National Institute of Infectious Diseases, Tokyo, Japan.
Arch Virol ; 165(10): 2335-2340, 2020 Oct.
Article in En | MEDLINE | ID: mdl-32719956
Sapoviruses are increasingly being recognized as pathogens associated with gastroenteritis in humans. Human sapoviruses are currently assigned to 18 genotypes (GI.1-7, GII.1-8, GIV.1, and GV.1-2) based on the sequence of the region encoding the major structural protein. In this study, we evaluated 11 polymerase chain reaction (PCR) assays using published and newly designed/modified primers and showed that four PCR assays with different primer combinations amplified all of the tested human sapovirus genotypes using either synthetic DNA or cDNA prepared from human sapovirus-positive fecal specimens. These assays can be used as improved broadly reactive screening tests or as tools for molecular characterization of human sapoviruses.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Viral Structural Proteins / DNA Primers / Caliciviridae Infections / Reverse Transcriptase Polymerase Chain Reaction / Sapovirus / Gastroenteritis Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Arch Virol Year: 2020 Document type: Article Affiliation country: Country of publication:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Viral Structural Proteins / DNA Primers / Caliciviridae Infections / Reverse Transcriptase Polymerase Chain Reaction / Sapovirus / Gastroenteritis Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Arch Virol Year: 2020 Document type: Article Affiliation country: Country of publication: