Method for identification of heme-binding proteins and quantification of their interactions.
Anal Biochem
; 607: 113865, 2020 10 15.
Article
in En
| MEDLINE
| ID: mdl-32738209
ABSTRACT
The standard assay for characterization of interaction of heme with proteins is absorbance spectroscopy. However, this approach demands relatively large quantities of proteins and it is difficult to perform in high-throughput manner. Here, we describe an immunosorbent assay based on the covalent in situ conjugation of heme to a pre-coated carrier. Advantage of this assay is that it allows both identification of heme-binding proteins and quantification of their binding avidity, using only minimal amounts of protein (1-10⯵g). Importantly, the same approach can be used for covalent linkage of other natural or synthetic compounds and analyzing their interactions with proteins.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Hemoglobins
/
Immunoglobulin G
/
Heme
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Anal Biochem
Year:
2020
Document type:
Article
Affiliation country: