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In vitro toxicity of isolated strains and cyanobacterial bloom biomasses over Paramecium caudatum (ciliophora): Lessons from a non-metazoan model organism.
Boas, Layne do Amaral Vilas; Senra, Marcus Vinicius Xavier; Fernandes, Kelly; Gomes, Andreia Maria da Anunciação; Pedroso Dias, Roberto Júnio; Pinto, Ernani; Fonseca, Ana Lúcia.
Affiliation
  • Boas LDAV; Instituto de Recursos Naturais, Universidade Federal de Itajubá, CEP 37500-903, Itajubá, Minas Gerais, Brazil.
  • Senra MVX; Instituto de Recursos Naturais, Universidade Federal de Itajubá, CEP 37500-903, Itajubá, Minas Gerais, Brazil; Laboratório de Protozoologia, Programa de Pós-graduação Em Comportamento e Biologia Animal, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Campus Universitário, CEP
  • Fernandes K; Faculty of Pharmaceutical Sciences, University of São Paulo, 05508-000, São Paulo, Brazil.
  • Gomes AMDA; Instituto Federal de Educação, Ciências e Tecnologia Do Rio de Janeiro, 24310-000, Niterói, Rio de Janeiro, Brazil.
  • Pedroso Dias RJ; Laboratório de Protozoologia, Programa de Pós-graduação Em Comportamento e Biologia Animal, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Campus Universitário, CEP 36036-900, Juiz de Fora, Minas Gerais, Brazil.
  • Pinto E; Faculty of Pharmaceutical Sciences, University of São Paulo, 05508-000, São Paulo, Brazil; Centre for Nuclear Energy in Agriculture, University of São Paulo, 13416-000, Piracicaba, São Paulo, Brazil; Food Research Center (FoRC - CEPID), University of São Paulo, 05508-000, São Paulo, Brazil.
  • Fonseca AL; Instituto de Recursos Naturais, Universidade Federal de Itajubá, CEP 37500-903, Itajubá, Minas Gerais, Brazil. Electronic address: afonseca@unifei.edu.br.
Ecotoxicol Environ Saf ; 202: 110937, 2020 Oct 01.
Article in En | MEDLINE | ID: mdl-32800220
ABSTRACT
Cyanobacteria have been considered a major global threat because of their widespread ability to proliferate and contaminate inland and marine waters with toxic metabolites. For this reason, to avoid risks to humans and environmental health, regulatory legislation and guidelines have been established based on extensive toxicological data. However, most of what is known in this field come from works on microcystin (MC) variants, which effects were almost exclusively tested in metazoan models. In this work, we used acute end-point toxicological assays and high-resolution hybrid quadrupole time-of-flight mass spectrometer coupled with electrospray ionization source (ESI-Q-TOF-MS) analyses to evaluate the deleterious impact of aqueous extracts prepared from cultures of cyanobacteria and environmental bloom biomasses over a non-metazoan model organism, the cosmopolitan fresh/brackish water unicellular microeukaryote, Paramecium caudatum (Ciliophora). Our data suggest that all extracts produced time-dependent effects on P. caudatum survival, irrespective of their metabolite profile; and that this ciliate is more sensitive to extracts containing microginins than to extracts with only MCs, stressing that more toxicological investigations should be performed on the environmental impact of neglected cyanotoxins. Further, our data provide evidence that P. caudatum may be more sensitive to cyanotoxins than vertebrates, indicating that guidelines values, set on metazoans are likely to be inaccurate to protect organisms from basal food web positions. Thus, we highly recommend the widespread use of microeukaryotes, such as ciliates in environmental risk assessment frameworks for the establishment of more reliable cyanotoxin monitoring guideline values.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cyanobacteria / Paramecium caudatum Type of study: Risk_factors_studies Limits: Animals / Humans Language: En Journal: Ecotoxicol Environ Saf Year: 2020 Document type: Article Affiliation country:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cyanobacteria / Paramecium caudatum Type of study: Risk_factors_studies Limits: Animals / Humans Language: En Journal: Ecotoxicol Environ Saf Year: 2020 Document type: Article Affiliation country:
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