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Bovine Leukemia Virus Infection Affects Host Gene Expression Associated with DNA Mismatch Repair.
Bai, Lanlan; Hirose, Tomoya; Assi, Wlaa; Wada, Satoshi; Takeshima, Shin-Nosuke; Aida, Yoko.
Affiliation
  • Bai L; Photonics Control Technology Team, RIKEN Center for Advanced Photonics, Wako 351-0198, Japan.
  • Hirose T; Viral Infectious Diseases Unit, RIKEN, Wako 351-0198, Japan.
  • Assi W; Viral Infectious Diseases Unit, RIKEN, Wako 351-0198, Japan.
  • Wada S; Laboratory of Viral Infectious Diseases, Department of Medical Genome Sciences, Graduate School of Frontier Science, The University of Tokyo, Tokyo 113-8657, Japan.
  • Takeshima SN; Photonics Control Technology Team, RIKEN Center for Advanced Photonics, Wako 351-0198, Japan.
  • Aida Y; Viral Infectious Diseases Unit, RIKEN, Wako 351-0198, Japan.
Pathogens ; 9(11)2020 Oct 30.
Article in En | MEDLINE | ID: mdl-33143351
ABSTRACT
Bovine leukemia virus (BLV) causes enzootic bovine leukosis, a malignant form of B-cell lymphoma, and is closely related to human T-cell leukemia viruses. We investigated whether BLV infection affects host genes associated with DNA mismatch repair (MMR). Next-generation sequencing of blood samples from five calves experimentally infected with BLV revealed the highest expression levels of seven MMR genes (EXO1, UNG, PCNA, MSH2, MSH3, MSH6, and PMS2) at the point of peak proviral loads (PVLs). Furthermore, MMR gene expression was only upregulated in cattle with higher PVLs. In particular, the expression levels of MSH2, MSH3, and UNG positively correlated with PVL in vivo. The expression levels of all seven MMR genes in pig kidney-15 cells and the levels of PMS2 and EXO1 in HeLa cells also increased tendencies after transient transfection with a BLV infectious clone. Moreover, MMR gene expression levels were significantly higher in BLV-expressing cell lines compared with those in the respective parental cell lines. Expression levels of MSH2 and EXO1 in BLV-infected cattle with lymphoma were significantly lower and higher, respectively, compared with those in infected cattle in vivo. These results reveal that BLV infection affects MMR gene expression, offering new candidate markers for lymphoma diagnosis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Risk_factors_studies Language: En Journal: Pathogens Year: 2020 Document type: Article Affiliation country:

Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Risk_factors_studies Language: En Journal: Pathogens Year: 2020 Document type: Article Affiliation country: