Proteins involved in oxidative stress in leiomyoma tissues treated with ulipristal acetate.
Mol Med Rep
; 23(1)2021 01.
Article
in En
| MEDLINE
| ID: mdl-33179092
ABSTRACT
Uterine leiomyoma presents the highest incidence among benign tumors of the female reproductive tract. The present study compared the proteome of leiomyoma treated with ulipristal acetate with that of untreated leiomyoma to investigate protein expression patterns in relation to oxidative stress. Paired tissue samples from seven treated and untreated leiomyomas were collected and the proteome was analyzed by twodimensional gel electrophoresis (2DE). Western blotting was used to validate the results of 2DE, and mass spectrometry was used to identify proteins. The tissue expression of 30 proteins was markedly affected by treatment with ulipristal acetate. Bioinformatics analysis revealed that several of the differentially expressed proteins were involved in the degradation of hydrogen peroxide and the synthesis of reactive oxygen species. The present study suggested the involvement of oxidative stress as a novel mechanism of action of ulipristal acetate. These findings require further investigations to understand the role of ulipristal acetate in the treatment of the leiomyoma.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Uterine Neoplasms
/
Proteomics
/
Gene Regulatory Networks
/
Leiomyoma
/
Norpregnadienes
Type of study:
Prognostic_studies
Limits:
Adult
/
Female
/
Humans
Language:
En
Journal:
Mol Med Rep
Year:
2021
Document type:
Article
Affiliation country: