Single-Cell Infrared Microspectroscopy Quantifies Dynamic Heterogeneity of Mesenchymal Stem Cells during Adipogenic Differentiation.
Anal Chem
; 93(2): 671-676, 2021 01 19.
Article
in En
| MEDLINE
| ID: mdl-33290049
ABSTRACT
The central relevance of cellular heterogeneity to biological phenomena raises the rational needs for analytical techniques with single-cell resolution. Here, we developed a single-cell FTIR microspectroscopy-based method for the quantitative evaluation of cellular heterogeneity by calculating the cell-to-cell similarity distance of the infrared spectral data. Based on this method, we revealed the infrared phenotypes might reflect the dynamic heterogeneity changes in the cell population during the adipogenic differentiation of the human mesenchymal stem cells. These findings provide an alternative label-free optical approach for quantifying the cellular heterogeneity, and the combination with other single-cell analysis tools will be very helpful for understanding the genotype-to-phenotype relationship in cellular populations.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Microspectrophotometry
/
Cell Differentiation
/
Adipogenesis
/
Single-Cell Analysis
/
Mesenchymal Stem Cells
Limits:
Humans
Language:
En
Journal:
Anal Chem
Year:
2021
Document type:
Article
Affiliation country: