Protocol for brain-wide or region-specific microglia depletion and repopulation in adult mice.
STAR Protoc
; 1(3): 100211, 2020 12 18.
Article
in En
| MEDLINE
| ID: mdl-33377105
ABSTRACT
The advent of tools enabling the direct manipulation of microglia has furthered our understanding of their role in health and disease. Here, we present a detailed protocol allowing for microglia turnover in adult CX3CR1creERT2 × iDTR or CX3CR1creERT2 × iDTR × tdTomatoflox mice, either in a brain-wide or region-specific manner, and their subsequent isolation for downstream applications. This protocol may be used to explore microglia biology and their putative region-specific heterogeneous functional diversity, expanding our understanding of their importance in various neuroinflammatory conditions. For complete details on the use and execution of this protocol, please refer to Willis et al. (2020).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Microglia
/
Flow Cytometry
Limits:
Animals
Language:
En
Journal:
STAR Protoc
Year:
2020
Document type:
Article
Affiliation country: