Prevalence of plasmid-mediated resistance genes among multidrug-resistant uropathogens in Egypt.
Afr Health Sci
; 20(1): 190-198, 2020 Mar.
Article
in En
| MEDLINE
| ID: mdl-33402907
ABSTRACT
BACKGROUND:
The emergence of multidrug-resistant (MDR) uropathogens has become a public health threat and current knowledge of the genotypic basis of bacterial resistance is essential for selecting appropriate treatment options.OBJECTIVES:
To determine the prevalence of antimicrobial resistance among MDR uropathogens and to elucidate the molecular bases of plasmid-mediated resistance.METHODS:
Bacterial isolates were recovered from urine specimens of 150 out-patients with signs and symptoms of urinary tract infections (UTIs) at El-Demerdash Hospital, Cairo, Egypt. Standard methods were used for identification, antimicrobial susceptibility testing was performed according to CLSI guidelines.RESULTS:
Among the recovered isolates, 22.7% and 77.3% were Gram-positive, and negative, respectively. Of which; 43.3% were MDR with 60% harboring plasmids. Extended spectrum ß-lactamase (ESBL) genes bla CTX-M, bla SHV, and bla TEM were detected on plasmids of 89.7%, 41%, and 84.6% of the tested isolates, respectively. The aminoglycoside resistance gene aac6'-Ib/aac-6'-Ib-cr was found on plasmids of 92.3% of the tested isolates followed by qnrS (92.3%), qnrB (46.2%), and qnrA (7.7%). The most prevalent quinolone efflux pump gene was oqxB (38.5%), followed by oqxA (20.5%), then qepA (10.3%).CONCLUSION:
High levels of resistance to nitrofurans, ß-lactam/ß-lactamase inhibitor, cephalosporins, aminoglycosides, and fluoroquinolones were detected, and their use as empirical treatment for UTIs has become questionable.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plasmids
/
Bacterial Proteins
/
Urinary Tract Infections
/
Gram-Positive Bacterial Infections
/
Gram-Negative Bacterial Infections
/
Drug Resistance, Multiple, Bacterial
/
Gram-Negative Bacteria
/
Gram-Positive Bacteria
/
Anti-Bacterial Agents
Type of study:
Guideline
/
Prevalence_studies
Limits:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Country/Region as subject:
Africa
Language:
En
Journal:
Afr Health Sci
Journal subject:
MEDICINA
/
SERVICOS DE SAUDE
Year:
2020
Document type:
Article
Affiliation country: