Uropathogenic E. coli induces DNA damage in the bladder.
PLoS Pathog
; 17(2): e1009310, 2021 02.
Article
in En
| MEDLINE
| ID: mdl-33630958
ABSTRACT
Urinary tract infections (UTIs) are among the most common outpatient infections, with a lifetime incidence of around 60% in women. We analysed urine samples from 223 patients with community-acquired UTIs and report the presence of the cleavage product released during the synthesis of colibactin, a bacterial genotoxin, in 55 of the samples examined. Uropathogenic Escherichia coli strains isolated from these patients, as well as the archetypal E. coli strain UTI89, were found to produce colibactin. In a murine model of UTI, the machinery producing colibactin was expressed during the early hours of the infection, when intracellular bacterial communities form. We observed extensive DNA damage both in umbrella and bladder progenitor cells. To the best of our knowledge this is the first report of colibactin production in UTIs in humans and its genotoxicity in bladder cells.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides
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Urinary Tract Infections
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Urinary Bladder
/
DNA Damage
/
Escherichia coli Infections
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Uropathogenic Escherichia coli
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Polyketides
Limits:
Aged
/
Animals
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Female
/
Humans
/
Male
Language:
En
Journal:
PLoS Pathog
Year:
2021
Document type:
Article
Affiliation country: