Cloning and expression analysis of GATA1 gene in Carassius auratus red var.
BMC Genom Data
; 22(1): 12, 2021 03 18.
Article
in En
| MEDLINE
| ID: mdl-33736593
ABSTRACT
BACKGROUND:
GATA1 is a key transcription factor in the GATA family, and promotes the differentiation and maturation of red blood cell, which is essential for normal hematopoiesis.RESULTS:
Our results showed that the cDNA sequence of GATA1 was 2730 bp long encoding 443 amino acids. qRT-PCR analysis demonstrated that GATA1 had the highest expression in testis (T), followed by pituitary (P) and spleen (S). GATA1 gene expression in C. auratus red var. embryo from the neuroblast stage (N) to the embryo hatching (H) changes continuously; and the gene expression levels of nonylphenol (NP)-treated and those of control embryos were significantly different. Moreover, Methylation levels of GATA1 gene in NP-treated embryos were higher than those in control embryos, indicating that NP affected GATA1 methylation.CONCLUSIONS:
Our study provides cues for further studying the roles of GATA1 gene in fish development, and suggested a potential molecular mechanism by which NP leads to abnormal development of fish embryos.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Goldfish
/
Cloning, Molecular
/
Gene Expression Profiling
/
GATA1 Transcription Factor
Limits:
Animals
Language:
En
Journal:
BMC Genom Data
Year:
2021
Document type:
Article