Disease-inducing potential of two leukemic cell lines in a xenografting model.
J Assist Reprod Genet
; 38(6): 1589-1600, 2021 Jun.
Article
in En
| MEDLINE
| ID: mdl-33786735
ABSTRACT
PURPOSE:
Cryopreserved ovarian tissue transplant restores ovarian function in young cancer patients after gonadotoxic treatment. However, leukemia is associated with increased risk of malignant cell transmission. We aimed to assess the tumor-inducing potential of two different leukemic cell lines when xenografted to immunodeficient mice.METHODS:
Fifty-four female immunodeficient mice were grafted with either 100, 200, 500, 1000, and 10,000 chronic myeloid leukemia in blast crisis (BV-173) cells or relapsed acute lymphoblastic leukemia (RCH-ACV) cells, embedded inside a fibrin scaffold along with 50,000 human ovarian stromal cells. Two mice per cell line received the fibrin matrix without leukemic cells as negative controls. Clinical signs of disease were monitored for 20 weeks. Grafts, liver tissue, and masses were collected for macroscopic analysis and gene expression of BCR-ABL1 and E2A-PBX fusion transcripts present in BV-173 and RCH-ACV respectively.RESULTS:
BV-173 cells Mice grafted with 100, 200, or 500 cells showed no sign of disease after and were negative for BCR-ABL1 expression. Three of the 5 animals grafted with 1000 cells and all mice with 10,000 cells developed disease and showed BCR-ABL1-positive expression. RCH-ACV cells Two out of 4 mice grafted with 100 cells developed disease and were E2A-PBX1-positive. All the animals grafted with higher cell doses showed signs of disease and all but one were E2A-PBX1-positive.CONCLUSION:
The present work proves that the disease-inducing potential of BV-173 and RCH-ACV leukemic cells xenografted to SCID mouse peritoneum differs between cell lines, depending on cell number, type, status, and cytogenetic disease profile when ovarian tissue is harvested.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transplantation, Heterologous
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive
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Precursor Cell Lymphoblastic Leukemia-Lymphoma
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Ovarian Follicle
Limits:
Animals
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Female
/
Humans
Language:
En
Journal:
J Assist Reprod Genet
Journal subject:
GENETICA
/
MEDICINA REPRODUTIVA
Year:
2021
Document type:
Article
Affiliation country: