THE EFFECT OF DIET ENRICHED WITH PYROPHOSPHATE (E450) ON EXPRESSION OF GENES ENCODING BONE MORPHOGENETIC PROTEIN AND OSTEOCALCIN IN MOUSE EMBRYONIC MANDIBLE TISSUES.
Wiad Lek
; 74(1): 43-47, 2021.
Article
in En
| MEDLINE
| ID: mdl-33851585
ABSTRACT
OBJECTIVE:
Theaim:
Of our study was to measure the mRNA expression of the investigated odontogenesis factors in mandible tissue of mouse embryos (17th day of pregnancy) gestated by females, kept on a E450 rich diet since 30 days before fertilization to gestation. PATIENTS ANDMETHODS:
Materials andmethods:
The effect of food supplements was studied in «Overload phosphates model¼. Experiments were carried out on white nonlinear outbred mice with mass 25-28g (n=40). The females from the control group were fed with standard rodent food, whereas the experimental females were fed with pyrophosphate-enriched food. The materials, used for the molecular genetic study, were the lower jaws of 17-days old mouse embryos (E-17).RESULTS:
Results:
The investigated BMP2 and osteocalcin genes are expressed at approximately the same level. Pyrophosphate-rich diet does not alter BMP2 gene expression, but it significantly increases the expression of osteocalcin.CONCLUSION:
Conclusions:
The present study is the first one to describe the impact of the pyrophosphate-rich diet on mRNA expression of key osteogenesis regulators - osteocalcin and BMP2.Key words
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Osteoblasts
/
Diphosphates
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Wiad Lek
Journal subject:
MEDICINA
Year:
2021
Document type:
Article
Affiliation country: