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Residential PM2.5 exposure and the nasal methylome in children.
Sordillo, Joanne E; Cardenas, Andres; Qi, Cancan; Rifas-Shiman, Sheryl L; Coull, Brent; Luttmann-Gibson, Heike; Schwartz, Joel; Kloog, Itai; Hivert, Marie-France; DeMeo, Dawn L; Baccarelli, Andrea A; Xu, Cheng-Jian; Gehring, Ulrike; Vonk, Judith M; Koppelman, Gerard; Oken, Emily; Gold, Diane R.
Affiliation
  • Sordillo JE; Division of Chronic Disease Research Across the Lifecourse, Department of Population Medicine, Harvard Medical School, and Harvard Pilgrim Health Care Institute, Boston, MA, USA.
  • Cardenas A; Division of Environmental Health Sciences, School of Public Health, University of California, Berkeley, 2121 Berkeley Way, Berkeley, CA, USA.
  • Qi C; University of Groningen, University Medical Center Groningen, Department of Pediatric Pulmonology and Pediatric Allergy, Beatrix Children's Hospital, Groningen, the Netherlands; University of Groningen, University Medical Center Groningen, Groningen Research Institute for Asthma and COPD (GRIAC), Gr
  • Rifas-Shiman SL; Division of Chronic Disease Research Across the Lifecourse, Department of Population Medicine, Harvard Medical School, and Harvard Pilgrim Health Care Institute, Boston, MA, USA.
  • Coull B; Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA, USA.
  • Luttmann-Gibson H; Department of Environmental Health, Harvard T. H. Chan School of Public Health, Boston, MA, USA.
  • Schwartz J; Department of Environmental Health, Harvard T. H. Chan School of Public Health, Boston, MA, USA.
  • Kloog I; Department of Environmental Health, Harvard T. H. Chan School of Public Health, Boston, MA, USA.
  • Hivert MF; Division of Chronic Disease Research Across the Lifecourse, Department of Population Medicine, Harvard Medical School, and Harvard Pilgrim Health Care Institute, Boston, MA, USA.
  • DeMeo DL; Channing Division of Network Medicine and Division of Pulmonary and Critical Care Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • Baccarelli AA; Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, NY, NY, USA.
  • Xu CJ; Research Group of Bioinformatics and Computational Genomics, CiiM, Centre for individualized infection medicine, a joint venture between Hannover Medical School and the Helmholtz Centre for Infection Research, Hannover, Germany; Department of Gastroenterology, Hepatology and Endocrinology, TWINCORE,
  • Gehring U; Institute for Risk Assessment Sciences, Utrecht University, Utrecht, the Netherlands.
  • Vonk JM; University of Groningen, University Medical Center Groningen, Groningen Research Institute for Asthma and COPD (GRIAC), Groningen, the Netherlands; University of Groningen, University Medical Center Groningen, Department of Epidemiology, Groningen, the Netherlands.
  • Koppelman G; University of Groningen, University Medical Center Groningen, Department of Pediatric Pulmonology and Pediatric Allergy, Beatrix Children's Hospital, Groningen, the Netherlands; University of Groningen, University Medical Center Groningen, Groningen Research Institute for Asthma and COPD (GRIAC), Gr
  • Oken E; Division of Chronic Disease Research Across the Lifecourse, Department of Population Medicine, Harvard Medical School, and Harvard Pilgrim Health Care Institute, Boston, MA, USA.
  • Gold DR; Department of Environmental Health, Harvard T. H. Chan School of Public Health, Boston, MA, USA; Channing Division of Network Medicine and Division of Pulmonary and Critical Care Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA. Electronic addre
Environ Int ; 153: 106505, 2021 08.
Article in En | MEDLINE | ID: mdl-33872926
ABSTRACT
RATIONALE PM2.5-induced adverse effects on respiratory health may be driven by epigenetic modifications in airway cells. The potential impact of exposure duration on epigenetic alterations in the airways is not yet known.

OBJECTIVES:

We aimed to study associations of fine particulate matter PM2.5 exposure with DNA methylation in nasal cells.

METHODS:

We conducted nasal epigenome-wide association analyses within 503 children from Project Viva (mean age 12.9 y), and examined various exposure durations (1-day, 1-week, 1-month, 3-months and 1-year) prior to nasal sampling. We used residential addresses to estimate average daily PM2.5 at 1 km resolution. We collected nasal swabs from the anterior nares and measured DNA methylation (DNAm) using the Illumina MethylationEPIC BeadChip. We tested 719,075 high quality autosomal CpGs using CpG-by-CpG and regional DNAm analyses controlling for multiple comparisons, and adjusted for maternal education, household smokers, child sex, race/ethnicity, BMI z-score, age, season at sample collection and cell-type heterogeneity. We further corrected for bias and genomic inflation. We tested for replication in a cohort from the Netherlands (PIAMA).

RESULTS:

In adjusted analyses, we found 362 CpGs associated with 1-year PM2.5 (FDR < 0.05), 20 CpGs passing Bonferroni correction (P < 7.0x10-8) and 10 Differentially Methylated Regions (DMRs). In 445 PIAMA participants (mean age 16.3 years) 11 of 203 available CpGs replicated at P < 0.05. We observed differential DNAm at/near genes implicated in cell cycle, immune and inflammatory responses. There were no CpGs or regions associated with PM2.5 levels at 1-day, 1-week, or 1-month prior to sample collection, although 2 CpGs were associated with past 3-month PM2.5.

CONCLUSION:

We observed wide-spread DNAm variability associated with average past year PM2.5 exposure but we did not detect associations with shorter-term exposure. Our results suggest that nasal DNAm marks reflect chronic air pollution exposure.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Air Pollution / Epigenome Limits: Adolescent / Child / Humans Country/Region as subject: Europa Language: En Journal: Environ Int Year: 2021 Document type: Article Affiliation country:
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Air Pollution / Epigenome Limits: Adolescent / Child / Humans Country/Region as subject: Europa Language: En Journal: Environ Int Year: 2021 Document type: Article Affiliation country: