Your browser doesn't support javascript.
loading
Novel decorated nanostructured lipid carrier for simultaneous active targeting of three anti-cancer agents.
Mahoutforoush, Amin; Solouk, Atefeh; Hamishehkar, Hamed; Haghbin Nazarpak, Masoumeh; Abbaspour-Ravasjani, Soheil.
Affiliation
  • Mahoutforoush A; Department of Biomedical Engineering, Central Tehran Branch, Islamic Azad University, Tehran 13185/768, Iran.
  • Solouk A; Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran 1591634311, Iran.
  • Hamishehkar H; Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: hamishehkar.hamed@gmail.com.
  • Haghbin Nazarpak M; New Technologies Research Center (NTRC), Amirkabir University of Technology, Tehran 1591634653, Iran.
  • Abbaspour-Ravasjani S; Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Life Sci ; 279: 119576, 2021 Aug 15.
Article in En | MEDLINE | ID: mdl-33965376
Cancer-targeted co-delivery of therapeutic agents has been recognized as an effective strategy for increasing efficacy and reducing side effects of therapeutic agents. In this study, we used methotrexate (MTX) alone as a targeting moiety and chemotherapeutic agent and in combination with docetaxel (DTX) and doxorubicin (DOX) as chemotherapeutic agents to stop cancer cell proliferation with the aid of newly designed nanostructured lipid carriers (NLCs). The physicochemical properties of our designed nanocomplexes were evaluated by DLS, FT-IR spectroscopy, SEM, and TEM. Moreover, the targeting efficiency of the designed and synthesized nanoplatforms was evaluated on the folate receptor (FR) positive human breast cancer cell line (MCF-7) and FR negative human alveolar basal epithelial cells (A549). The NLCs/DTX/DOX/CS and NLCs/DTX/DOX/CS-MTX complexes significantly increased the cell cytotoxicity and the cell apoptosis rate. However, the complexes significantly reduced the capability of colony formation and cell migration. Our results revealed that NLCs/DTX/DOX/CS-MTX had synergistic cytotoxicity, reactive oxygen spaces, autophagy, and the apoptosis induction ability with an enhanced cellular internalization rate in FR-positive cancer cells, thorough MTX recognition capability. We conclude that the NLCs/DTX/DOX/CS-MTX complex is a new promising paradigm for breast cancer-targeted co-delivery.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Breast Neoplasms / Drug Carriers / Antineoplastic Combined Chemotherapy Protocols / Nanostructures / Lipids Limits: Female / Humans Language: En Journal: Life Sci Year: 2021 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Breast Neoplasms / Drug Carriers / Antineoplastic Combined Chemotherapy Protocols / Nanostructures / Lipids Limits: Female / Humans Language: En Journal: Life Sci Year: 2021 Document type: Article Affiliation country: Country of publication: