Between life and death: strategies to reduce phototoxicity in super-resolution microscopy.
J Phys D Appl Phys
; 53(16): 163001, 2020 Apr 15.
Article
in En
| MEDLINE
| ID: mdl-33994582
ABSTRACT
Super-resolution microscopy (SRM) enables non-invasive, molecule-specific imaging of the internal structure and dynamics of cells with sub-diffraction limit spatial resolution. One of its major limitations is the requirement for high-intensity illumination, generating considerable cellular phototoxicity. This factor considerably limits the capacity for live-cell observations, particularly for extended periods of time. Here, we give an overview of new developments in hardware, software and probe chemistry aiming to reduce phototoxicity. Additionally, we discuss how the choice of biological model and sample environment impacts the capacity for live-cell observations.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Type of study:
Prognostic_studies
Language:
En
Journal:
J Phys D Appl Phys
Year:
2020
Document type:
Article
Affiliation country: