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An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells.
Baglia, Regina A; Mills, Kira R; Mitra, Koushambi; Tutol, Jasmine N; Ball, Darby; Page, Kierstin M; Kallu, Jyothi; Gottipolu, Sriharika; D'Arcy, Sheena; Nielsen, Steven O; Dodani, Sheel C.
Affiliation
  • Baglia RA; Department of Chemistry and Biochemistry, The University of Texas at Dallas Richardson TX 75080 USA sheel.dodani@utdallas.edu.
  • Mills KR; Department of Chemistry and Biochemistry, The University of Texas at Dallas Richardson TX 75080 USA sheel.dodani@utdallas.edu.
  • Mitra K; Department of Chemistry and Biochemistry, The University of Texas at Dallas Richardson TX 75080 USA sheel.dodani@utdallas.edu.
  • Tutol JN; Department of Chemistry and Biochemistry, The University of Texas at Dallas Richardson TX 75080 USA sheel.dodani@utdallas.edu.
  • Ball D; Department of Chemistry and Biochemistry, The University of Texas at Dallas Richardson TX 75080 USA sheel.dodani@utdallas.edu.
  • Page KM; Department of Chemistry and Biochemistry, The University of Texas at Dallas Richardson TX 75080 USA sheel.dodani@utdallas.edu.
  • Kallu J; Department of Chemistry and Biochemistry, The University of Texas at Dallas Richardson TX 75080 USA sheel.dodani@utdallas.edu.
  • Gottipolu S; Department of Chemistry and Biochemistry, The University of Texas at Dallas Richardson TX 75080 USA sheel.dodani@utdallas.edu.
  • D'Arcy S; Department of Chemistry and Biochemistry, The University of Texas at Dallas Richardson TX 75080 USA sheel.dodani@utdallas.edu.
  • Nielsen SO; Department of Chemistry and Biochemistry, The University of Texas at Dallas Richardson TX 75080 USA sheel.dodani@utdallas.edu.
  • Dodani SC; Department of Chemistry and Biochemistry, The University of Texas at Dallas Richardson TX 75080 USA sheel.dodani@utdallas.edu.
RSC Chem Biol ; 2(3): 830-834, 2021 Mar 15.
Article in En | MEDLINE | ID: mdl-34212150
Human phenol sulfotransferases mediate the transfer of a sulfuryl moiety from the activated sulfate donor PAPS to hydroxy-containing substrates, altering substrate solubility and charge to affect phase II metabolism and cell signaling. Here, we present the development, computational modeling, in vitro enzymology, and biological application of STS-3, an activity-based fluorescent sensor for the SULT1A1 isoform.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Diagnostic_studies Language: En Journal: RSC Chem Biol Year: 2021 Document type: Article Country of publication:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Diagnostic_studies Language: En Journal: RSC Chem Biol Year: 2021 Document type: Article Country of publication: