Bioanalytical RP-HPLC method for the determination of ponazuril in plasma.
Biomed Chromatogr
; 35(12): e5210, 2021 Dec.
Article
in En
| MEDLINE
| ID: mdl-34216023
ABSTRACT
The goal of this investigation was to establish a reliable technique for the quantitation of ponazuril in limited sample volumes. Samples were extracted with chloroform and separation was achieved with a Symmetry RP18 column. Ultraviolet absorption was measured at 254 nm. A combination of 0.1% formic acid and acetonitrile (5050) was used as the mobile phase. The calibration curve was linear from 0.1-25 µg/mL, with a lower limit of quantification of 0.1 µg/mL with a 100 µL sample. The precision and accuracy were well within the range set by the Food and Drug Administration and the recovery was over 95%. This technique was used to analyze ponazuril samples and found to be appropriate for pharmacokinetic studies.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Triazines
/
Chromatography, High Pressure Liquid
/
Chromatography, Reverse-Phase
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Biomed Chromatogr
Year:
2021
Document type:
Article
Affiliation country: