Production of MSTN-mutated cattle without exogenous gene integration using CRISPR-Cas9.
Biotechnol J
; 17(7): e2100198, 2022 Jul.
Article
in En
| MEDLINE
| ID: mdl-34247443
ABSTRACT
Many genome-edited animals have been produced using clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 technology to edit specific genes. However, there are few guidelines for the application of this technique to cattle. The goal of this study was to produce trait-improved cattle using the genome-editing technology CRISPR-Cas9. Myostatin (MSTN) was selected as a target locus, and synthetic mRNA of sgRNA and Cas9 were microinjected into fertilized bovine embryos in vitro. As a result, 17 healthy calves were born, and three of them showed MSTN mutation rates of 10.5%, 45.4%, and 99.9%, respectively. Importantly, the offspring with the 99.9% MSTN mutation rate had a biallelic mutation (-12 bps) and a double-muscling phenotype. In conclusion, we demonstrate that the genome-editing technology CRISPR-Cas9 can produce genetically modified calves with improved traits.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Myostatin
/
CRISPR-Cas Systems
Limits:
Animals
Language:
En
Journal:
Biotechnol J
Journal subject:
BIOTECNOLOGIA
Year:
2022
Document type:
Article