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Novel DNA methylation markers of PRRSV-specific antibodies and their intergenerational transmission from pregnant sows to piglets.
Zhang, Fengxia; Chen, Siqian; Yang, Ting; Ao, Hong; Zhai, Liwei; Li, Qianjun; Xing, Kai; Liu, Yibing; Liu, Huatao; Yu, Ying; Wang, Chuduan.
Affiliation
  • Zhang F; National Engineering Laboratory for Animal Breeding, MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, China; Innovation Team of Pig Health Breeding, Institute of Animal Husbandry and Veterinary, Tianjin Academy of
  • Chen S; National Engineering Laboratory for Animal Breeding, MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, China.
  • Yang T; National Engineering Laboratory for Animal Breeding, MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, China.
  • Ao H; The State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
  • Zhai L; National Engineering Laboratory for Animal Breeding, MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, China.
  • Li Q; Innovation Team of Pig Health Breeding, Institute of Animal Husbandry and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin, China.
  • Xing K; Animal Science and Technology College, Beijing University of Agriculture, Beijing, China.
  • Liu Y; National Engineering Laboratory for Animal Breeding, MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, China.
  • Liu H; National Engineering Laboratory for Animal Breeding, MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, China.
  • Yu Y; National Engineering Laboratory for Animal Breeding, MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, China. Electronic address: yuying@cau.edu.cn.
  • Wang C; National Engineering Laboratory for Animal Breeding, MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, China. Electronic address: cdwang@cau.edu.cn.
Gene ; 801: 145831, 2021 Oct 30.
Article in En | MEDLINE | ID: mdl-34274485
ABSTRACT
The main strategy for preventing porcine reproductive and respiratory syndrome (PRRS) is vaccination. However, current commercial porcine reproductive and respiratory syndrome virus (PRRSV) vaccines have limited effectiveness and may even cause infections in pigs. The identification of stable molecular markers associated with immune responses to PRRSV vaccination in pigs provides a new approach for PRRS prevention. DNA methylation, the most stable epigenetic molecular marker related to PRRSV vaccination, has not been investigated. In the current research, we used whole genome bisulfite sequencing (WGBS) to investigate DNA methylation in pregnant sows that received PRRSV vaccination and their piglets with high and low PRRSV-specific antibody levels. By performing methylation data analysis and basing on our previous transcriptomic studies, we identified several differentially methylated genes (DMGs) that are involved in the pathways of inflammatory and immune responses. Among the DMGs, ISG15, MX1, SERPINE1, GNG11 and IFIT3 were common hub genes in the two generations. MX1 and GNG11 were located in quantitative trait loci related with PRRSV antibody titer and PRRSV susceptibility, respectively. These results suggest that PRRSV vaccination in sows induces DNA methylation changes in genes and DNA methylation changes occur through intergenerational transmission. The novel DNA methylation markers and target genes observed in our study provide new insights into the molecular mechanisms of immune responses to PRRSV vaccination across two pig generations.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Viral Vaccines / Porcine Reproductive and Respiratory Syndrome / DNA Methylation / Antibodies, Viral Type of study: Prognostic_studies Limits: Animals / Pregnancy Language: En Journal: Gene Year: 2021 Document type: Article Publication country: HOLANDA / HOLLAND / NETHERLANDS / NL / PAISES BAJOS / THE NETHERLANDS
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Viral Vaccines / Porcine Reproductive and Respiratory Syndrome / DNA Methylation / Antibodies, Viral Type of study: Prognostic_studies Limits: Animals / Pregnancy Language: En Journal: Gene Year: 2021 Document type: Article Publication country: HOLANDA / HOLLAND / NETHERLANDS / NL / PAISES BAJOS / THE NETHERLANDS