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Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex.
Wang, Qiang; Guan, Zeyuan; Qi, Liangbo; Zhuang, Jinjin; Wang, Chen; Hong, Sixing; Yan, Ling; Wu, Yan; Cao, Xiaoqian; Cao, Jianbo; Yan, Junjie; Zou, Tingting; Liu, Zhu; Zhang, Delin; Yan, Chuangye; Yin, Ping.
Affiliation
  • Wang Q; National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China.
  • Guan Z; National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China.
  • Qi L; National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China.
  • Zhuang J; National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China.
  • Wang C; National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China.
  • Hong S; National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China.
  • Yan L; National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China.
  • Wu Y; National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China.
  • Cao X; National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China.
  • Cao J; Public Laboratory of Electron Microscopy, Huazhong Agricultural University, Wuhan 430070, China.
  • Yan J; National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China.
  • Zou T; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
  • Liu Z; National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China.
  • Zhang D; National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China.
  • Yan C; Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
  • Yin P; National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China.
Science ; 373(6561): 1377-1381, 2021 Sep 17.
Article in En | MEDLINE | ID: mdl-34446444
ABSTRACT
ß barrel outer membrane proteins (ß-OMPs) play vital roles in mitochondria, chloroplasts, and Gram-negative bacteria. Evolutionarily conserved complexes such as the mitochondrial sorting and assembly machinery (SAM) mediate the assembly of ß-OMPs. We investigated the SAM-mediated assembly of the translocase of the outer membrane (TOM) core complex. Cryo­electron microscopy structures of SAM­fully folded Tom40 and the SAM-Tom40/Tom5/Tom6 complexes at ~3-angstrom resolution reveal that Sam37 stabilizes the mature Tom40 mainly through electrostatic interactions, thus facilitating subsequent TOM assembly. These results support the ß barrel switching model and provide structural insights into the assembly and release of ß barrel complexes.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Carrier Proteins / Mitochondrial Membrane Transport Proteins / Multiprotein Complexes / Mitochondrial Membranes Limits: Humans Language: En Journal: Science Year: 2021 Document type: Article Affiliation country:
Fulltext
Add to My VHL
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Carrier Proteins / Mitochondrial Membrane Transport Proteins / Multiprotein Complexes / Mitochondrial Membranes Limits: Humans Language: En Journal: Science Year: 2021 Document type: Article Affiliation country: