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Analysis of codon-specific translation by ribosome profiling.
Kim, Yeji; Eggers, Cristian; Shvetsova, Ekaterina; Kleemann, Leon; Sin, Olga; Leidel, Sebastian A.
Affiliation
  • Kim Y; University of Bern, Department of Chemistry, Biochemistry and Pharmaceutical Sciences, Bern, Switzerland.
  • Eggers C; University of Bern, Department of Chemistry, Biochemistry and Pharmaceutical Sciences, Bern, Switzerland.
  • Shvetsova E; University of Bern, Department of Chemistry, Biochemistry and Pharmaceutical Sciences, Bern, Switzerland.
  • Kleemann L; University of Bern, Department of Chemistry, Biochemistry and Pharmaceutical Sciences, Bern, Switzerland.
  • Sin O; University of Bern, Department of Chemistry, Biochemistry and Pharmaceutical Sciences, Bern, Switzerland.
  • Leidel SA; University of Bern, Department of Chemistry, Biochemistry and Pharmaceutical Sciences, Bern, Switzerland. Electronic address: sebastian.leidel@unibe.ch.
Methods Enzymol ; 658: 191-223, 2021.
Article in En | MEDLINE | ID: mdl-34517947
ABSTRACT
Chemical modifications of RNA molecules can affect translation in multiple ways. Therefore, it is critical to understand how their absence changes cellular translation dynamics and in particular codon-specific translation. In this chapter, we discuss the application of ribosome profiling to analyze changes in codon-specific translation and differential translation in Saccharomyces cerevisiae and human cells.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Protein Biosynthesis / RNA, Transfer Limits: Humans Language: En Journal: Methods Enzymol Year: 2021 Document type: Article Affiliation country:
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Protein Biosynthesis / RNA, Transfer Limits: Humans Language: En Journal: Methods Enzymol Year: 2021 Document type: Article Affiliation country: