Microbiome of Pukzing Cave in India shows high antimicrobial activity against plant and animal pathogens.
Genomics
; 113(6): 4098-4108, 2021 11.
Article
in En
| MEDLINE
| ID: mdl-34699904
ABSTRACT
Pukzing cave, the largest cave of Mizoram, India was explored for bacterial diversity. Culture dependent method revealed 235 bacterial isolates using three different treatments. Identity of the microbial species was confirmed by 16S rDNA sequencing. The highest bacterial population was recovered from heat treatment (n = 97;41.2%) followed by normal (n = 79;33.6%) and cold treatment (n = 59;25.1%) indicating dominance of moderate thermophiles. Antimicrobial potential of isolates showed 20.4% isolates having antimicrobial ability against tested pathogens. Amplicon sequencing of PKSI, PKSII and NRP specific genes revealed presence of AMP genes in the microbial population. Six microbial pathogens were selected for screening as they are well known for different disease cause organism in various fields such as agriculture and human health. Cave environment harbors unique microbial flora and hypervariable region V4 is more informative. Higher activity of AMP assay against these microbes indicates that cave microbial communities could be potential source of future genomic resources.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Microbiota
Limits:
Animals
Language:
En
Journal:
Genomics
Journal subject:
GENETICA
Year:
2021
Document type:
Article
Affiliation country: