Production of 10-Hydroxy-2-decenoic Acid from Decanoic Acid via Whole-Cell Catalysis in Engineered Escherichia coli.
ChemSusChem
; 15(9): e202102152, 2022 May 06.
Article
in En
| MEDLINE
| ID: mdl-34796684
ABSTRACT
10-Hydroxy-2-decenoic acid (10-HDA) is a terminal hydroxylated medium-chain α,ß-unsaturated carboxylic acid that performs various unique physiological activities and has a wide market value. Therefore, development of an environmentally friendly, safe, and high-efficiency route to synthesize 10-HDA is required. Here, the ß-oxidation pathway of Escherichia coli was modified and a P450 terminal hydroxylase (CYP153A33-CPRBM3 ) was rationally designed to synthesize 10-HDA using decanoic acid as a substrate via two-step whole-cell catalysis. Different homologues of FadDs, FadEs, and YdiIs were analyzed in the first step of the conversion of decanoic acid to trans- -2- decenoic acid. In the second step, CYP153A33 (M228L)-CPRBM3 efficiently catalyzed the conversion of trans- -2- decenoic acid to 10-HDA. Finally, 217â
mg L-1 10-HDA was obtained with 500â
mg L-1 decanoic acid. This study provides a strategy for biosynthesis of 10-HDA and other α, ß-unsaturated carboxylic acid derivatives from specific fatty acids.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carboxylic Acids
/
Escherichia coli
Language:
En
Journal:
ChemSusChem
Journal subject:
QUIMICA
/
TOXICOLOGIA
Year:
2022
Document type:
Article