LncRNA HOXC-AS3 increases non-small cell lung cancer cell migration and invasion by sponging premature miR-96.
Expert Rev Respir Med
; 16(5): 587-593, 2022 05.
Article
in En
| MEDLINE
| ID: mdl-35034519
ABSTRACT
BACKGROUND:
Long noncoding RNA (lncRNA) HOXC cluster antisense RNA 3 (HOXC-AS3) has been involved in breast cancer and gastric cancer, while its role in non-small cell lung cancer (NSCLC) is unknown.METHODS:
The expression of HOXC-AS3 and miR-96 (both mature and premature) were detected using RT-qPCR. Nuclear fractionation assay and RNA pull-down assay were performed to detect the subcellular location of HOXC-AS3 and potential interaction with premature miR-96, respectively. Overexpression assays were performed to determine the role of HOXC-AS3 in the maturation of miR-96. Transwell assays were performed to explore the role of HOXC-AS3 and miR-96 in NSCLC cell invasion and migration.RESULTS:
NSCLC tissues exhibited significantly increased expression levels of HOXC-AS3 and premature miR-96. HOXC-AS3 was localized to both nucleus and cytoplasm, and a direct interaction between HOXC-AS3 and premature miR-96 was observed. In NSCLC cells, HOXC-AS3 upregulated the expression of premature miR-96 but downregulated the expression of mature miR-96. Moreover, HOXC-AS3 suppressed the role of miR-96 in inhibiting NSCLC cell invasion and migration.CONCLUSION:
HOXC-AS3 may increase NSCLC cell growth and invasion by sponging premature miR-96 to suppress its maturation.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carcinoma, Non-Small-Cell Lung
/
MicroRNAs
/
RNA, Long Noncoding
/
Lung Neoplasms
Limits:
Humans
Language:
En
Journal:
Expert Rev Respir Med
Year:
2022
Document type:
Article