A new murine ileostomy model: recycling stool prevents intestinal atrophy in the distal side of ileostomy.
Fujita Med J
; 7(2): 41-49, 2021.
Article
in En
| MEDLINE
| ID: mdl-35111543
ABSTRACT
OBJECTIVES:
Proximal stoma creation in neonates results in growth failure and distal intestinal atrophy. "Recycling stool" consists of stool injection from the proximal limb to the distal limb of a stoma. Because this method may prevent distal bowel atrophy and increase body weight, we investigated the effects of recycling stool upon distal intestinal mucosa by generating an ileostomy model in rats.METHODS:
An ileostomy was created 5 cm proximal to the cecum in male Wistar/ST rats. Discharged stool or saline was injected into the distal limb, twice per day for 7 days. The intestinal adaptation was assessed by measuring the villus height and counting goblet cell number. Proliferation and apoptosis were analyzed by Ki67 and TUNEL immunostaining.RESULTS:
The ratios of the height of the distal villi (D) to the that of proximal villi (P) were 0.97 (median [range] of D and P length 421 [240-729] µm and 436 [294-638] µm, P<0.05) in the stool-injected group and 0.81 in the saline-injected group (442 [315-641] µm and 548 [236-776] µm, P<0.05). Compared with the saline-injected group, the stool-injected group showed elevated numbers of goblet cells (3.6 [2.0-7.6] vs. 4.9 [2.4-7.5] cells/100-µm villus length) and Ki67-positive cells (26.8% [13.8%-35.4%] vs. 40.1% [31.2%-45.7%]), along with a reduced number of apoptotic cells (5.0 [2.0-14.0] vs. 4.0 [1.0-9.0] cells/100-µm villus length).CONCLUSIONS:
Recycling stool prevented distal intestinal atrophy; this experimental design may facilitate further studies concerning alternative methods to prevent intestinal atrophy and growth failure.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Fujita Med J
Year:
2021
Document type:
Article
Affiliation country: