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Exosomal miR-483-5p in Bone Marrow Mesenchymal Stem Cells Promotes Malignant Progression of Multiple Myeloma by Targeting TIMP2.
Gu, Jianmei; Wang, Maoye; Wang, Xinfeng; Li, Jiao; Liu, Haiyan; Lin, Zenghua; Yang, Xi; Zhang, Xu; Liu, Hong.
Affiliation
  • Gu J; Department of Hematology, First Affiliated Hospital of Soochow University, Suzhou, China.
  • Wang M; Department of Clinical Laboratory Medicine, Affiliated Cancer Hospital of Nantong University, Nantong, China.
  • Wang X; Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China.
  • Li J; Department of Hematology, Affiliated Hospital of Nantong University, Nantong, China.
  • Liu H; Department of Hematology, First Affiliated Hospital of Soochow University, Suzhou, China.
  • Lin Z; Department of Hematology, Affiliated Hospital of Nantong University, Nantong, China.
  • Yang X; Department of Hematology, Affiliated Hospital of Nantong University, Nantong, China.
  • Zhang X; Department of Hematology, Affiliated Hospital of Nantong University, Nantong, China.
  • Liu H; Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China.
Front Cell Dev Biol ; 10: 862524, 2022.
Article in En | MEDLINE | ID: mdl-35300408
ABSTRACT
Bone marrow-derived mesenchymal stem cell (BMSC) is one crucial component of the multiple myeloma (MM) microenvironment and supports the malignant progression of MM. Whether BMSCs act on MM cells via exosomes has not been well characterized. Herein, we used microarrays to screen out differentially expressed miRNAs in BMSCs from patients with MM (MM-MSCs) or benign diseases (BD-MSCs). We found that miR-483-5p was highly expressed in MM-MSCs, which may be transported through exosomes from MM-MSCs to MM cells to increase miR-483-5p expression in them. We then investigated the role and mechanism of miR-483-5p in the aggressive progression of MM in vitro. We verified that miR-483-5p promoted MM cell proliferation and reduced apoptosis. Then we predicted and validated that TIMP2, a tumor suppressor gene, is the downstream target of miR-483-5p in MM. In summary, our study indicated that MM-MSCs promote MM malignant progression via the release of exosomes and regulation of miR-483-5p/TIMP2 axis, suggesting an essential role of BMSCs derived exosomal miRNA in MM and a potential marker for MM diagnosis and therapy.
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Front Cell Dev Biol Year: 2022 Document type: Article Affiliation country:

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Front Cell Dev Biol Year: 2022 Document type: Article Affiliation country:
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