[Molecular analysis of 23 cases of B subgroup].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
; 39(5): 546-547, 2022 May 10.
Article
in Zh
| MEDLINE
| ID: mdl-35598276
ABSTRACT
OBJECTIVE:
To explore the molecular reasons of weak expression of B antigen on the red cell.METHODS:
Serological test for blood group was carried out, including red cell and plasma grouping, and anti-A1 and anti-H testing, and confirming weak A or B antigens by adsorption and elution. Exons 1-7 were sequenced directly, and one of them was cloned and sequenced.RESULTS:
All of the 23 samples showed the weak B antigen by serological method. The alleles of the subgroups were identified by DNA sequencing, including 2 Bel subgroup, 4 B3 subgroup, 14 Bw subgroup, 2 CisAB subgroup and a novel allele. The novel allele showed a nucleotide substitution 662G>A in the exon 7, and the sequence was submitted to Blood Group Antigen Gene Mutation Database, and the novel allele was named Bel10.CONCLUSION:
Nucleotide substitution in exon results in blood subgroup, which showed that the antigens were weakened, and Bw phenotype was the most frequently subgroup.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
ABO Blood-Group System
/
Nucleotides
Type of study:
Prognostic_studies
Limits:
Humans
Language:
Zh
Journal:
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
Journal subject:
GENETICA MEDICA
Year:
2022
Document type:
Article
Affiliation country: