Flow cytometry analysis of endothelial cells and subsets of exhausted CD8+ T cells in murine tumor models.
STAR Protoc
; 3(2): 101444, 2022 06 17.
Article
in En
| MEDLINE
| ID: mdl-35677615
ABSTRACT
Here, we present a protocol for flow cytometry analysis of endothelial cells (ECs) and CD8+ T cells in murine tumor models, at baseline and after cancer immunotherapy with anti-PD-1/anti-CTLA-4 antibodies. We provide gating strategies for identification of specific cell subsets including ECs from tumor-associated high endothelial venules (TA-HEVs), stem-like, and terminally exhausted CD8+ T cells. This protocol represents a valuable tool for the analysis of rare subsets of tumor ECs and CD8+ T cells with critical roles in antitumor immunity. For complete details on the use and execution of this protocol, please refer to Asrir et al. (2022).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Programmed Cell Death 1 Receptor
/
Neoplasms
Limits:
Animals
Language:
En
Journal:
STAR Protoc
Year:
2022
Document type:
Article
Affiliation country: