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Identification of apple cultivars hypoallergenic for birch pollen-allergic individuals by a multidisciplinary in vitro and in vivo approach.
Strobl, Maria R; Vollmann, Ute; Eckl-Dorna, Julia; Radakovics, Astrid; Ibl, Verena; Schnurer, Madeleine; Brenner, Martin; Dermendjiev, Georgi; Weckwerth, Wolfram; Neumüller, Michael; Frommlet, Florian; Demir, Hilal; Bublin, Merima; Müller, Christian; Bohle, Barbara.
Affiliation
  • Strobl MR; Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Wien, Austria.
  • Vollmann U; Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Wien, Austria.
  • Eckl-Dorna J; Department of Otorhinolaryngology, Medical University of Vienna, Vienna, Austria.
  • Radakovics A; Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Wien, Austria.
  • Ibl V; Department of Functional and Evolutionary Ecology, Division of Molecular Systems Biology, Faculty of Life Sciences, University of Vienna, Vienna, Austria.
  • Schnurer M; Department of Functional and Evolutionary Ecology, Division of Molecular Systems Biology, Faculty of Life Sciences, University of Vienna, Vienna, Austria.
  • Brenner M; Department of Functional and Evolutionary Ecology, Division of Molecular Systems Biology, Faculty of Life Sciences, University of Vienna, Vienna, Austria.
  • Dermendjiev G; Department of Functional and Evolutionary Ecology, Division of Molecular Systems Biology, Faculty of Life Sciences, University of Vienna, Vienna, Austria.
  • Weckwerth W; Department of Functional and Evolutionary Ecology, Division of Molecular Systems Biology, Faculty of Life Sciences, University of Vienna, Vienna, Austria.
  • Neumüller M; Bavarian Centre of Pomology and Fruit Breeding, Hallbergmoos, Germany.
  • Frommlet F; Center for Medical Statistics, Informatics and Intelligent Systems, Section for Medical Statistics, Medical University of Vienna, Vienna, Austria.
  • Demir H; Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Wien, Austria.
  • Bublin M; Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Wien, Austria.
  • Müller C; Department of Otorhinolaryngology, Medical University of Vienna, Vienna, Austria.
  • Bohle B; Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Wien, Austria.
Clin Transl Allergy ; 12(8): e12186, 2022 Aug.
Article in En | MEDLINE | ID: mdl-36036236
ABSTRACT

BACKGROUND:

Birch pollen-related apple allergy is the most frequent IgE-mediated food allergy in Central-Northern Europe with Mal d 1 as major allergen. Its concentration in apples varies with the cultivar and storage time. Year-round appealing, hypoallergenic cultivars still are needed to satisfy the nutritional needs of affected individuals. We characterized three promising cultivars by multidisciplinary in vitro assays including long-term storage and by clinical challenges of allergic individuals before and after the birch pollen season.

METHODS:

Proteins were extracted from fruits of 'Santana', 'Golden Delicious' (GD), and three genuine cultivars in November 2018 and April 2019. Mal d 1-levels were analysed by mass spectrometry, SDS-PAGE, immunoblotting, competitive ELISA, and basophil activation tests. Twenty-eight allergic individuals underwent single-blinded open food challenges and skin testing with the cultivars and birch pollen in November 2018 and May 2019. Allergen-specific IgE-levels were determined.

RESULTS:

After storage all cultivars except 'Santana' were of appealing appearance and taste. Their Mal d 1 content had increased, also reflected by significantly amplified basophil activation and stronger reactions in clinical challenges. Besides, individuals showed boosted reactivity after pollen exposure indicated by enhanced allergen-specific IgE-levels and skin reactions to birch pollen. Still, all cultivars remained significantly less allergenic than GD and comparable to Santana in November 2018 in all assessments except for skin testing.

CONCLUSIONS:

Combined expertise in pomology and allergology identified promising new cultivars for allergic consumers. The evaluation of hypoallergenic apples should incorporate long-term storage and birch pollen exposure. Basophil activation tests may be suitable in the selection of promising cultivars for oral challenges.
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Diagnostic_studies Language: En Journal: Clin Transl Allergy Year: 2022 Document type: Article Affiliation country:

Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Diagnostic_studies Language: En Journal: Clin Transl Allergy Year: 2022 Document type: Article Affiliation country: