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Measuring Endocannabinoid System Interaction with Biomembranes.
Angelucci, Clotilde B; Sabatucci, Annalaura; Bernardo, Ana Lia; Kurtz, Alexandrine; Oddi, Sergio; Dainese, Enrico.
Affiliation
  • Angelucci CB; Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
  • Sabatucci A; Faculty of Bioscience and Technology for Food Agriculture and Environment, University of Teramo, Teramo, Italy.
  • Bernardo AL; Faculty of Bioscience and Technology for Food Agriculture and Environment, University of Teramo, Teramo, Italy.
  • Kurtz A; Faculty of Bioscience and Technology for Food Agriculture and Environment, University of Teramo, Teramo, Italy.
  • Oddi S; Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
  • Dainese E; European Center for Brain Research/Santa Lucia Foundation IRCCS, Rome, Italy.
Methods Mol Biol ; 2576: 425-436, 2023.
Article in En | MEDLINE | ID: mdl-36152207
ABSTRACT
Understanding the correct interaction among the different components of the endocannabinoid (eCB) system is fundamental for a proper assessment of the function of eCBs as signaling molecules. The knowledge of how the membrane environment modulates the intracellular trafficking of the eCB system and its interacting proteins holds a huge potential in unraveling new mechanisms of its modulation. This chapter deals with the application of fluorescence resonance energy transfer technique to measure the binding affinity of eCB proteins to model membranes (i.e., large unilamellar vesicles, LUVs). In particular, we describe in detail the paradigmatic example of the interaction of rat recombinant fatty acid amide hydrolase with LUVs constituted of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Endocannabinoids / Unilamellar Liposomes Limits: Animals Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2023 Document type: Article Affiliation country:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Endocannabinoids / Unilamellar Liposomes Limits: Animals Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2023 Document type: Article Affiliation country:
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